Identification of in vitro and in vivo oncolytic effect in colorectal cancer cells by Orf virus strain NA1/11.
Identification of in vitro and in vivo oncolytic effect in colorectal cancer cells by Orf virus strain NA1/11.
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Orf病毒株NA1/11对结直肠癌细胞的体外和体内溶瘤作用鉴定
DOI：
10.3892/or.2020.7885
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发表时间：
2021-03
期刊：
影响因子：
4.2
通讯作者：
Hao W
中科院分区：
文献类型：
--
作者：
Chen D;Wang R;Long M;Li W;Xiao B;Deng H;Weng K;Gong D;Liu F;Luo S;Hao W
ORFV （ORFV）是研究中较好的溶瘤病毒载体，ORFV菌株NZ2在免疫调节谱介导的动物模型中具有抗肿瘤作用。然而，ORFV在结直肠癌（CRC）细胞中引发的抗肿瘤作用尚不清楚。通过western blotting、菌落形成、CCK-8、创面划伤实验、qPCR和动物模型来确定ORFV在结直肠癌中的体内和体外作用。通过细胞因子抗体芯片实验、流式细胞术、western blotting和免疫组化（IHC）等方法探讨ORFV的潜在发病机制。结果表明，ORFV菌株NA1/11感染并抑制结直肠癌细胞的体外生长和迁移。通过建立Balb/c小鼠CRC模型，发现ORFV菌株NA1/11显著抑制CRC细胞在体内的生长和迁移。利用细胞因子抗体阵列获得更全面的描述，揭示ORFV感染中差异表达的细胞因子。细胞因子如IL-7、IL-13、IL-15、CD27、CD30、戊烷素3和B淋巴细胞化学引诱剂（BLC）上调。Axl、CXCL16、ANG-3、MMP10、IFN-γ R1、VEGF-B下调。结果表明，ORFV在细胞凋亡、自身免疫/炎症、血管生成和细胞周期等关键因子的调控中发挥作用。最后，数据验证了ORFV感染通过增强体内和体外细胞凋亡诱导溶瘤活性。总之，ORFV可能是一种用于结直肠癌治疗的溶瘤病毒。
Orf virus (ORFV) is a favorable oncolytic viral carrier in research, and ORFV strain NZ2 has been revealed to have antitumor effects in animal models mediated by immunoregulation profile. However, the antitumor effects triggered by the ORFV in colorectal cancer (CRC) cells is poorly characterized. The in vivo and in vitro roles of ORFV in CRC were determined using western blotting, colony formation, CCK-8, wound scratch assay, qPCR, and animal models. Furthermore, cytokine antibody chip assay, flow cytometry, western blotting, and immunohistochemical (IHC) assays were conducted to explore the potential mechanism of ORFV. The present data revealed that ORFV strain NA1/11 infected and inhibited the in vitro growth and migration of CRC cells. By establishing a CRC model in Balb/c mice, it was revealed that ORFV strain NA1/11 significantly inhibited the in vivo growth and migration of CRC cells. A cytokine antibody array was utilized to obtain a more comprehensive profile revealing the differentially expressed cytokines in ORFV infection. Cytokines, such as IL-7, IL-13, IL-15, CD27, CD30, pentraxin 3, and B lymphocyte chemoattractant (BLC), were upregulated. Axl, CXCL16, ANG-3, MMP10, IFN-γ R1 and VEGF-B were downregulated. The results indicated that ORFV played roles in the regulation of key factors relevant to apoptosis, autoimmunity/inflammation, angiogenesis, and the cell cycle. Finally, data was presented to validate that ORFV infection induces oncolytic activity by enhancing apoptosis in vivo and in vitro. In conclusion, ORFV could be an oncolytic virus for CRC therapy.
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影响因子：
11.2
作者：
Croce CM;Reed JC
通讯作者：
Reed JC
影响因子：
3.1
作者：
LaBarre, DD;Lowy, RJ
通讯作者：
Lowy, RJ
影响因子：
7
作者：
Huang, Z. M.;Du, S. H.;Tong, P.
通讯作者：
Tong, P.
影响因子：
4.4
作者：
Abel, S;Hundhausen, C;Ludwig, A
通讯作者：
Ludwig, A
影响因子：
3.8
作者：
Friebe, Astrid;Friederichs, Sonja;Webert, Olaf
通讯作者：
Webert, Olaf