Administration of the insulin into the scaffold atelocollagen for tissue-engineered cartilage
Administration of the insulin into the scaffold atelocollagen for tissue-engineered cartilage
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DOI：
10.1002/jbm.a.33046
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发表时间：
2011-05-01
影响因子：
4.9
通讯作者：
Hoshi, Kazuto
中科院分区：
文献类型：
--
作者：
Ko, Edward Chengchuan;Fujihara, Yuko;Hoshi, Kazuto
组织工程软骨结构的三维培养可能会增加基质的产生，但如果结构变大，必然会发生中心坏死。为了增加构建体中部的细胞活力并增强体内软骨再生，我们尝试将胰岛素注入支架中。已知胰岛素可以强烈增强软骨细胞中的基质生成。将含有去端肽胶原水凝胶的人耳软骨细胞沉淀在培养基中进行 3D 培养。三组（去端胶原中混合胰岛素、培养基中添加胰岛素以及对照组，即既不加入去端胶原也不加入胰岛素）的比较表明，混合在去端胶原和培养基中的胰岛素均能有效促进软骨细胞的细胞活力和基质合成。每日检测还显示胰岛素从去端肽胶原水凝胶中逐渐释放，表明这种材料可以作为胰岛素的控制释放。实际上，我们将携带软骨细胞和与或不与胰岛素混合的去端胶原的聚-L-丙交酯多孔支架移植到裸鼠体内，结果表明，在有胰岛素的组中，糖胺聚糖的积累很明显，尽管没有胰岛素的组中糖胺聚糖的积累较少。因此，我们表明，当将胰岛素注射到去端肽胶原水凝胶中时，可以增强体内软骨再生。 (C) 2011 Wiley periodicals, Inc. J Biomed Mater Res Part A: 97A: 186-192, 2011。
Three-dimensional culture of the tissue-engineered cartilage constructs may increase the matrix production, but central necrosis must occur if the construct becomes large. To increase the cell viability in the middle part of constructs and to enhance the in vivo cartilage regeneration, we attempted to administer the insulin into the scaffold. Insulin is known to strongly enhance the matrix production in the chondrocytes. The pellets of human auricular chondrocytes with atelocollagen hydrogel were 3D-cultured in the medium. The comparison among three groups (insulin mixed in the atelocollagen, insulin added to the medium, and control group, i.e.; insulin in neither atelocollagen nor medium) revealed that both insulin mixed in the atelocollagen and that in the medium could effectively promoted the cell viability and matrix synthesis of the chondrocytes. The daily assay also showed the gradual release of insulin from the atelocollagen hydrogel, suggesting that this material may work as a control release of insulin. We actually transplanted the poly-L-lactide porous scaffolds carrying the chondrocytes and the atelocollagen mixed with or without insulin, into the nude mice, showing that glycosaminoglycan accumulation was evident in the group with insulin although less without insulin. We thus showed the possibility to enhance the in vivo cartilage regeneration, when administered insulin into the atelocollagen hydrogel. (C) 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 97A: 186-192, 2011.