Humoral immune reactivity to feline leukemia virus and associated antigens in cats naturally infected with feline leukemia virus.
Humoral immune reactivity to feline leukemia virus and associated antigens in cats naturally infected with feline leukemia virus.
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发表时间：
1980-10
期刊：
影响因子：
11.2
通讯作者：
H. Lutz;N. Pedersen;J. Higgins;U. Hübscher;F. Troy;G. Theilen
中科院分区：
文献类型：
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作者：
H. Lutz;N. Pedersen;J. Higgins;U. Hübscher;F. Troy;G. Theilen
通过酶联免疫吸附测定法对自然感染猫白血病病毒（FeLV）的猫的体液抗体反应进行了检测。持续带毒的猫所产生的抗体水平低于从感染中康复的猫。在康复前2至36周带毒的猫，其酶联免疫吸附测定值变化不定，与非带毒猫和持续带毒猫的值有重叠。从FeLV感染中康复的猫的血清中，针对FeLV的抗体水平与针对猫肿瘤病毒相关细胞膜抗原的抗体之间有很强的相关性，但在持续带毒猫的血清中则没有。通过凝胶电泳和酶联免疫吸附测定相结合的方法对持续带毒和康复的猫的血清进行了分析，该方法能够对纯化的FeLV的每种蛋白质成分的抗体水平进行定量。康复猫的血清中含有针对五种主要FeLV结构蛋白（gp73、p27、p15、p12、p10）以及针对另外八种表观分子量分别为58000、47000、36000、24000、17000、15700、12000和8500的抗原成分的抗体。主要的抗体反应性针对58000道尔顿的蛋白质。持续带毒猫的血清中也含有针对所有这些成分的抗体，但水平显著降低。17000和15700道尔顿的蛋白质与一种针对鼠白血病病毒p15（E）的单克隆抗体有交叉反应，47000道尔顿的蛋白质似乎在抗原性上与肌动蛋白有关。根据与针对gag蛋白的特异性血清的交叉反应判断，58000、36000和24000道尔顿的种类含有gag基因同源性。发现猫肿瘤病毒相关细胞膜抗原参考血清对p15（E）表现出高反应性。结论是，不仅针对gp73，而且针对gag基因编码的蛋白质和多聚蛋白的特异性抗体在预防FeLV感染方面都很重要。
The humoral antibody response of cats infected naturally with feline leukemia virus (FeLV) was measured by an enzymelinked immunosorbent assay. Cats that became persistently viremic developed lower antibody levels than did cats which recovered from the infection. Cats that were viremic for 2 to 36 weeks prior to recovery had variable enzyme-linked immuno sorbent assay values that overlapped with the values seen in both nonviremic and persistently viremic cats. There was a strong correlation between antibody levels against FeLV and antibodies to feline oncornavirus-associated cell membrane antigen in sera from cats which recovered from FeLV infection but not in sera from cats that were persistently viremic. Sera from persistently viremic and recovered cats were analyzed by a combination of gel electrophoresis and enzymelinked immunosorbent assay, a procedure which permitted the levels of antibodies to each of the protein components of purified FeLV to be quantitated. Sera from recovered cats contained antibodies reactive against the five major FeLV struc tural proteins (gp73, p27, p15, p12, p10) and against eight additional antigenic components with apparent molecular weight values of 58,000, 47,000, 36,000, 24,000, 17,000, 15,700, 12,000, and 8,500. The major antibody reactivity was directed against the 58,000-dalton protein. Sera from persist ently viremic cats also contained antibodies to all of these components but at a significantly reduced level. The 17,000- and 15,700-dalton proteins were cross-reactive with a monoclonal antibody to the p15(E) of murine leukemia virus, and the 47,000-dalton protein appeared to be antigenically related to actin. The 58,000-, 36,000-, and 24,000-dalton species contained gag gene homology as judged by their cross-reactivity with sera specific for gag proteins. Feline on cornavirus-associated cell membrane antigen reference sera were found to exhibit high reactivity against p15(E). It is concluded that antibodies specific for not only gp73 but also for gag gene-coded proteins and polyproteins are impor tant in protection against FeLV infection.