Gastro-protective effect of edible plant Artemisia argyi in ethanol-induced rats via normalizing inflammatory responses and oxidative stress
Gastro-protective effect of edible plant Artemisia argyi in ethanol-induced rats via normalizing inflammatory responses and oxidative stress
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食用植物艾叶通过使炎症反应和氧化应激正常化对乙醇诱导的大鼠胃保护作用
DOI：
10.1016/j.jep.2017.12.023
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发表时间：
2018-03-25
影响因子：
5.4
通讯作者：
Meng, Dali
中科院分区：
文献类型：
--
作者：
Li, Shuang;Zhou, Shaobo;Meng, Dali
药理学相关性：艾蒿是一种民族药，长期用于治疗胃病和胃病证。材料与方法：将60只大鼠随机分为对照组、模型组（乙醇诱导）、对照组（奥美拉唑治疗组）和艾蒿组，每组10只。艾叶提取物（AT）（0.3、0.1、0.033 g/mL）处理组。处死大鼠后测定组织、血清生化指标及胃内容物胃蛋白酶活性。采用LPS诱导的RAW264.7细胞进行抗炎实验。艾叶提取物通过调节炎症因子、超氧化物歧化酶和丙二醛的水平，对乙醇诱导的大鼠胃粘膜有较强的保护作用，其保护作用上级优于0.3 g/mL阳性对照。在抗氧化实验中，黄酮类化合物能下调LPS诱导的RAW264.7巨噬细胞的促炎细胞因子水平，并能清除自由基。甲氧基取代的杜仲苷和栀子苷活性最强，说明甲氧基对活性的影响很大。结论：艾叶可通过抑制炎症反应和改善氧化应激来减轻乙醇所致大鼠胃粘膜损伤. A.结论：艾蒿是一种营养价值较高的植物，适宜胃粘膜损伤或不良饮食习惯人群作为必需的膳食补充剂，将促进艾蒿的种植和应用。在农业和食品工业中使用。
Pharmacological relevance: Artemisia argyi, a kind of ethnic drug, has a long-term use on gastric diseases and syndromes.Aim of the study: The aim of the study is to validate the traditional uses of A. argyi scientifically and to discover more efficient nature derived gastro-protective ethnomedicine and further elucidate the possible mechanisms.Materials and methods: Sixty rats were randomly divided into control, model (ethanol-induced), reference (omeprazole-treated) and A. argyi extract (AT) (0.3, 0.1, 0.033 g/mL) treated groups, respectively. The levels of biochemical indexes in tissues and serum and the activities of pepsin in gastric contents were measured after the sacrifice of rats. Moreover, the anti-inflammatory effects in LPS-induced RAW 264.7 cells of the isolated compounds were determined.Results: The studies indicated that A. argyi extract could exert strong protective effects on gastric mucosa in ethanol-induced rat model by regulating the levels of inflammatory factors, superoxide dismutase, and malonaldehyde, which were superior to those of positive control at 0.3 g/mL. The isolated flavonoids could down regulate the levels of pro-inflammatory cytokines on LPS-induced RAW 264.7 macrophage cells and eliminate free radicals in the anti-oxidative tests. The effects of eupatilin and jaceosidin, which were substituted by additional methoxy groups, were predominant, indicting the importance of methoxy to the activities.Conclusion: The results confirmed that A. argyi can protect ethanol-induced rats from gastric mucosal injury through inhibiting inflammatory responses and ameliorating oxidative stress. A. argyi is suitable for people with gastric mucosal injuries or unhealthy dietary habits as a necessary dietary supplement, which will promote the planting and application of A. argyi in both agriculture and food industry.