Caudoputamen is damaged by hypocapnia during mechanical ventilation in a rat model of chronic cerebral hypoperfusion
Caudoputamen is damaged by hypocapnia during mechanical ventilation in a rat model of chronic cerebral hypoperfusion
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DOI：
10.1161/hs1201.100216
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发表时间：
2001-12-01
期刊：
影响因子：
8.3
通讯作者：
Shingu, K
中科院分区：
文献类型：
--
作者：
Miyamoto, E;Tomimoto, H;Shingu, K
背景和目的-术后脑功能障碍，如精神错乱，是麻醉的常见并发症，有时会延长，尤其是在脑血管疾病患者中。采用大鼠慢性脑低灌流模型，观察麻醉期间低碳酸血症对神经元损伤的影响。方法：夹闭雄性Wistar大鼠双侧颈总动脉造成慢性脑低灌流。手术后14天。这些动物被机械通风2小时，然后在合适的条件下再饲养14天。24只大鼠被分成4组：慢性脑低灌注组，麻醉期间低碳酸血症或正常碳酸血症；假手术组，低碳酸血症或正常碳酸血症。用Kluver-Barrera染色评价脑切片上的白质损害。用星形胶质细胞标志物--胶质纤维酸性蛋白免疫组织化学方法检测胶质细胞的增殖情况。小胶质细胞的标志物CD11b。计算机辅助形态计量学应用于微管相关蛋白2免疫组织化学结果以评估神经元的丢失。结果：慢性脑低灌注组大鼠组织学损伤几乎仅局限于白质，但无低碳酸血症。慢性脑低灌注伴低碳酸血症大鼠尾壳核白质损伤加重，神经元损伤和星形胶质细胞增殖。结论：这些结果表明，麻醉过程中低碳酸血症导致尾壳核组织损伤，这可能是中风和/或痴呆患者术后长期精神错乱的原因之一。
Background and Purpose-Postoperative brain dysfunction, such as delirium, is a common complication of anesthesia and is sometimes prolonged, especially in patients with cerebrovascular disease. In the present study we investigated the effect of hypocapnia during anesthesia on neuronal damage using a rat model of chronic cerebral hypoperfusion.Methods-Chronic cerebral hypoperfusion was induced by clipping the bilateral common carotid arteries in male Wistar rats. Fourteen days after the operation. these animals were mechanically ventilated For 2 hours and then kept in suitable conditions for an additional 14 days. Twenty-four rats were assigned to 4 groups: those with chronic cerebral hypoperfusion with either hypocapnia or normocapnia during anesthesia, and those given sham operation with either hypocapnia or normocapnia. White matter lesions in the brain sections were evaluated with Kluver-Barrera staining. Proliferation of glial cells was estimated with the use of immunohistochemistry of glial fibrillary acidic protein, a marker for astroglia. and CD11b, a marker for microglia. Computer-assisted morphometry was applied to the immunohisto- chemical results of microtubule-associated protein 2 to evaluate the loss of neurons.Results-The histological damage was localized almost exclusively in the white matter in the rats subjected to chronic cerebral hypoperfusion but without hypocapnia. Neuronal damage and astroglial proliferation occurred with aggravated white matter lesions in the caudoputamen in the rats with chronic cerebral hypoperfusion and hypocapnia. No lesions were observed in sham-operated rats with either hypocapnia or normocapnia.Conclusions-These results indicate that hypocapnia during anesthesia causes tissue damage in the caudoputamen, which may be responsible for long-lasting postoperative delirium in patients with stroke and/or dementia.