Increased sCD200 Levels in Vitreous of Patients With Proliferative Diabetic Retinopathy and Its Correlation With VEGF and Proinflammatory Cytokines
Increased sCD200 Levels in Vitreous of Patients With Proliferative Diabetic Retinopathy and Its Correlation With VEGF and Proinflammatory Cytokines
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增殖性糖尿病视网膜病变患者玻璃体内sCD200水平升高及其与VEGF和促炎细胞因子的相关性
DOI：
10.1167/iovs.15-16854
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发表时间：
2015-10-01
影响因子：
4.4
通讯作者：
Liang, Xiaoling
中科院分区：
文献类型：
--
作者：
Xu, Yue;Cheng, Qiaochu;Liang, Xiaoling
目的.本研究旨在探讨增殖性糖尿病视网膜病变（proliferative diabetic retinopathy，PDR）患者玻璃体中sCD 200的表达水平及其与玻璃体视网膜病变、VEGF及其受体和促炎细胞因子的关系。ELISA法检测sCD 200、VEGF及其受体和其他促炎细胞因子的表达。对不同玻璃体视网膜情况的患者进行临床分层，进行相关性分析。PDR组玻璃体sCD 200水平（182.2 ± 17.63 pg/mL）显著高于对照组（56.86 ± 6.573 pg/mL; P < 0.0001）。PDR组静脉血sCD 200水平为26.71 +/- 4.32 pg/mL，对照组为19.94 +/- 3.87 pg/mL（P = 0.2614）。PDR合并糖尿病性黄斑水肿患者玻璃体sCD 200水平显著升高（DME; 266.9 +/- 28.82 pg/mL）或牵引性视网膜脱离（TRD; 256.9 +/- 34.50 pg/mL）与无DME（136.9 +/- 15.13 pg/mL; P < 0.0001）或TRD（146.9 +/- 15.97 pg/mL; P = 0.0024）的PDR组相比。PDR组玻璃体中CCL 2、CXCL 4、CXCL 9、CXCL 10、VEGF、sVEGFR-1、sVEGFR-2、IL-6、IL-8、IL-10和IL-18水平也显著升高。sCD 200水平与VEGF之间存在统计学相关性（r = 0.6566，P < 0.0001），sVEGFR-1（r = 0.5574，P = 0.006），sVEGFR-2（r = 0.3605，P = 0.0362），CCL-2 IL-6（r = 0.5704，P = 0.0004）、IL-8（r = 0.3712，P = 0.0307）和IL-10（r = 0.3618，P = 0.0355）。sCD 200的表达可能通过与VEGF介导的炎症反应相互作用而促进视网膜血管生成，并代表PDR患者的潜在治疗靶点。
PURPOSE. The purpose of this study was to determine the levels of sCD200 expression in the vitreous of proliferative diabetic retinopathy (PDR) patients and to clarify its correlation with different vitreoretinal conditions, VEGF and its receptors, and proinflammatory cytokines.METHODS. The expression of sCD200, VEGF and its receptors, and other proinflammatory cytokines were examined by using ELISA. Clinical stratification was performed on patients with different vitreoretinal conditions for correlation analysis.RESULTS. The vitreous levels of sCD200 were significantly higher in the PDR group (182.2 +/- 17.63 pg/mL) compared with those in the control group (56.86 +/- 6.573 pg/mL; P < 0.0001). The venous blood levels of sCD200 were 26.71 +/- 4.32 pg/mL in the PDR group and 19.94 +/- 3.87 pg/mL in the control group (P = 0.2614). The vitreous levels of sCD200 were significantly elevated in PDR patients with diabetic macular edema (DME; 266.9 +/- 28.82 pg/mL) or traction retinal detachment (TRD; 256.9 +/- 34.50 pg/mL) compared with the PDR group without DME (136.9 +/- 15.13 pg/mL; P < 0.0001) or TRD (146.9 +/- 15.97 pg/mL; P = 0.0024). The vitreous levels of CCL2, CXCL4, CXCL9, CXCL10, VEGF, sVEGFR-1, sVEGFR-2, IL-6, IL-8, IL-10, and IL-18 were also elevated significantly in the PDR group. Statistical association was found between sCD200 levels and VEGF (r = 0.6566, P < 0.0001), sVEGFR-1 (r = 0.5574, P = 0.006), sVEGFR-2 (r = 0.3605, P = 0.0362), CCL-2 (r = 0.6001, P = 0.0002), IL-6 (r = 0.5704, P = 0.0004), IL-8 (r = 0.3712, P = 0.0307), and IL-10 (r = 0.3618, P = 0.0355).CONCLUSIONS. Expression of sCD200 may contribute to retinal angiogenesis by interacting with VEGF-mediated inflammatory response and represents a potential therapeutic target for the patients with PDR.