Production of the small heat shock protein Lo18 from Oenococcus oeni in Lactococcus lactis improves its stress tolerance.
Production of the small heat shock protein Lo18 from Oenococcus oeni in Lactococcus lactis improves its stress tolerance.
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DOI：
10.1016/j.ijfoodmicro.2016.06.005
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发表时间：
2017-04
影响因子：
5.4
通讯作者：
S. Weidmann;M. Maitre;J. Laurent;F. Coucheney;A. Rieu;J. Guzzo
中科院分区：
文献类型：
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作者：
S. Weidmann;M. Maitre;J. Laurent;F. Coucheney;A. Rieu;J. Guzzo
乳酸乳球菌是一种广泛用于奶酪和发酵乳生产的乳酸菌。在发酵过程中，L.乳酸菌受到酸性胁迫，从而损害其生长。来自嗜酸物种酒酒球菌的小热休克蛋白 (sHsp) Lo18 在 L 中表达。乳酸。已知该 sHsp 在 O 中的蛋白质保护和膜稳定中发挥重要作用。奥尼。可以在 L 中研究该 sHsp 的作用。乳酸菌，因为在该物种中没有检测到编码 sHsp 的基因。 lactissubsp.cremorisstrain MG1363 用 pDLhsp18 质粒转化，该质粒源自 pDL278，包含 hsp18 基因（编码 Lo18）及其自身的启动子序列。通过免疫印迹和 Lo18 inL 的细胞分布检查应激条件下 Lo18 的产生。测定热休克后的乳酸细胞。我们的结果清楚地表明 Lo18 在应激期间细胞质蛋白保护和膜稳定中的作用。 sHsp inL 的产生。乳酸提高了该物种对热和酸条件的耐受性。最后是L的改进。强调了 Lo18 在牛奶培养基中的乳酸存活，表明该 sHsp 具有有趣的作用。这些发现表明 aL 表达 sHsp。乳酸菌株具有更强的抗应激能力，因此可以增强工业菌株的性能。
Lactococcus lactis is a lactic acid bacterium widely used in cheese and fermented milk production. During fermentation,L. lactisis subjected to acid stress that impairs its growth. The small heat shock protein (sHsp) Lo18 from the acidophilic species Oenococcus oeni was expressed inL. lactis. This sHsp is known to play an important role in protein protection and membrane stabilization inO. oeni. The role of this sHsp could be studied inL. lactis,since no gene encoding for sHsp has been detected in this species.L. lactissubsp.cremorisstrain MG1363 was transformed with the pDLhsp18plasmid, which is derived from pDL278 and contains thehsp18gene (encoding Lo18) and its own promoter sequence. The production of Lo18 during stress conditions was checked by immunoblotting and the cellular distribution of Lo18 inL. lactiscells after heat shock was determined. Our results clearly indicated a role for Lo18 in cytoplasmic protein protection and membrane stabilization during stress. The production of sHsp inL. lactisimproved tolerance to heat and acid conditions in this species. Finally, the improvement of theL. lactissurvival in milk medium thanks to Lo18 was highlighted, suggesting an interesting role of this sHsp. These findings suggest that the expression of a sHsp by aL. lactisstrain results in greater resistance to stress, and, can consequently enhance the performances of industrial strains.