Measurement by fluorescence of interstitial adenosine levels in normoxic, hypoxic, and ischemic perfused rat hearts.
Measurement by fluorescence of interstitial adenosine levels in normoxic, hypoxic, and ischemic perfused rat hearts.
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通过荧光测量常氧、缺氧和缺血灌注大鼠心脏的间质腺苷水平。
DOI：
10.1161/01.res.60.2.177
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发表时间：
1987
影响因子：
20.1
通讯作者：
DobsonJr,JG
中科院分区：
文献类型：
--
作者：
Fenton,RA;DobsonJr,JG
用改良的方法研究了缺氧或缺血对离体非工作大鼠心脏组织间液和冠状静脉流出液中腺苷水平的影响。将5~10微升的左心外膜漏出液和冠脉流出液中的腺苷与氯乙醛反应，用高效液相色谱和荧光检测法测定其荧光衍生物1，N6-亚乙基腺苷的含量。对低氧有反应的心脏可分为两组。在一组心脏中，对照(常氧)漏出液和流出液的腺苷浓度分别为94+/-24和41+/-6pmol/ml。在低氧5分钟(30%O2)时，这些值分别增加了118和96%，并在常氧恢复5分钟后恢复到对照水平。在第二组心脏中，在低氧条件下，漏出液(42+/-7pmol/ml)和冠脉流出液(62+/-17pmol/ml)中腺苷的常氧控制水平分别增加了174%和1178%。然而，在恢复常氧灌流后，漏出液水平在5分钟内继续上升，而流出液水平下降。在另一组心脏中，全心缺血30秒可引起渗出液中腺苷水平比对照组(58+/-15 pmol/ml)高出362%至641%，这是在恢复血流30秒后确定的。
An improved assay was used to investigate the effects of hypoxia or ischemia on interstitial fluid and coronary venous effluent levels of adenosine in isolated perfused nonworking rat hearts. The adenosine in 5- to 10-microliter samples of left ventricular epicardial surface transudates and coronary effluents was reacted with chloroacetaldehyde, and the fluorescent derivative (1,N6-ethenoadenosine) was quantitated using high pressure liquid chromatography and fluorescence detection. Hearts responding to hypoxia could be separated into two groups. In one group of hearts, the control (normoxic) transudate and effluent adenosine concentrations were 94 +/- 24 and 41 +/- 6 pmol/ml, respectively. These values increased by 118 and 96%, respectively, with 5 minutes of hypoxia (30% O2), and returned to control levels 5 minutes after resumption of normoxia. In a second group of hearts, the normoxic control levels of adenosine in the transudates (42 +/- 7 pmol/ml) and coronary effluents (62 +/- 17 pmol/ml) were increased with hypoxia by 174 and 1,178%, respectively. However, the transudate levels continued to rise for 5 minutes after resumption of normoxic perfusion while effluent levels fell. In another series of hearts, global ischemia for 30 seconds elicited an elevation of transudate adenosine levels by 362 to 641% above control (58 +/- 15 pmol/ml) as determined 30 seconds after resumption of perfusion flow.(ABSTRACT TRUNCATED AT 250 WORDS)