COLONIC NEOPLASMS IN MICE PRODUCED WITH 6 INJECTIONS OF 1,2-DIMETHYLHYDRAZINE
COLONIC NEOPLASMS IN MICE PRODUCED WITH 6 INJECTIONS OF 1,2-DIMETHYLHYDRAZINE
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DOI：
10.1159/000225236
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发表时间：
1977-01-01
期刊：
影响因子：
3.5
通讯作者：
LONG, FC
中科院分区：
文献类型：
--
作者：
DESCHNER, EE;LONG, FC
报道了一种用于在CF 1雌性小鼠中诱导结肠癌的高度有效但减少的注射方案，其使用6次皮下注射。以20 mg/kg体重的剂量水平接种1，2-二甲基肼。在实验的第20周和第45周之间，83%的动物带有肿瘤，每只小鼠肉眼可见的结肠肿瘤的频率为2.1。肿瘤在性质上是孤立的，主要位于远端大肠。在30%的小鼠中发生了一定程度的结肠粘膜地毯状覆盖和无数肿瘤，这些融合肿瘤区域也发生在远端结肠。这种肿瘤的分布谱与在人类中观察到的相似，从而增强了这种时间保存的结肠肿瘤模型的价值。
A highly effective but reduced injection schedule for the induction of the colon cancer in CF1 female mice is reported using 6 s.c. inoculations of 1,2-dimethylhydrazine at a dose level of 20 mg/kg body weight. Between the 20th and 45th wk of the experiment, 83% of the animals were tumor-bearing with a frequency of 2.1 colonic neoplasms macroscopically visible per mouse. Tumors were isolated in nature and primarily located in the distal large bowel. Some degree of carpeting of the colonic mucosa and uncountable numbers of tumors occurred in 30% of mice and these areas of confluent neoplasms also occurred in the distal colon. This spectrum of distribution of tumors parallels that seen in man, thus enhancing the value of this time conserving colon tumor model.