A temperature-sensitive FERONIA mutant allele that alters root hair growth
A temperature-sensitive FERONIA mutant allele that alters root hair growth
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DOI：
10.1093/plphys/kiaa051
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发表时间：
2021-02-01
期刊：
影响因子：
7.4
通讯作者：
Nielsen, Erik
中科院分区：
文献类型：
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作者：
Kim, Daewon;Yang, Jiyuan;Nielsen, Erik
在植物中，根毛经历称为尖端生长的高度极化的细胞扩张形式，其中细胞壁沉积仅限于根毛顶端。为了确定在早期的遗传筛选中可能错过的基本细胞成分，我们确定了条件温度敏感（TS）根毛突变体的甲基磺酸乙酯诱变拟南芥。在这里，我们描述了这些突变体之一，feronia-temperature sensitive（fer-ts）。突变体fer-ts幼苗在正常温度（20摄氏度）下不受影响，但在高温（30摄氏度）下无法形成根毛。图位克隆和全基因组测序结果表明，fer-ts是由FERONIA胞外区（FER）的G41 S取代引起的。含有fer-ts突变的FER的功能性荧光融合定位于质膜，但在升高的温度下受到增强的蛋白质周转。在正常温度下，在野生型和fer-ts突变体中加入快速碱化因子1（RALF 1）肽可以快速抑制根尖生长，而在高温下，fer-ts幼苗的根伸长对RALF 1肽具有抗性。此外，在升高的温度下，fer-ts幼苗生长素处理后，表现出改变活性氧（ROS）的积累和phenocopied组成的fer突变体的各种植物激素处理的反应。分子建模和序列比较与其他长春花受体样激酶1 L（CrRLK 1 L）受体家族成员显示，突变的甘氨酸在fer-ts是高度保守的，但不位于最近表征的RALF 23和LORELI样糖蛋白2结合结构域，可能表明fer-ts表型可能不是直接由于结合RALF 1肽的损失。
In plants, root hairs undergo a highly polarized form of cell expansion called tip-growth, in which cell wall deposition is restricted to the root hair apex. In order to identify essential cellular components that might have been missed in earlier genetic screens, we identified conditional temperature-sensitive (ts) root hair mutants by ethyl methanesulfonate mutagenesis in Arabidopsis thaliana. Here, we describe one of these mutants, feronia-temperature sensitive (fer-ts). Mutant fer-ts seedlings were unaffected at normal temperatures (20 degrees C), but failed to form root hairs at elevated temperatures (30 degrees C). Map based-cloning and whole-genome sequencing revealed that fer-ts resulted from a G41S substitution in the extracellular domain of FERONIA (FER). A functional fluorescent fusion of FER containing the fer-ts mutation localized to plasma membranes, but was subject to enhanced protein turnover at elevated temperatures. While tip-growth was rapidly inhibited by addition of rapid alkalinization factor 1 (RALF1) peptides in both wild-type and fer-ts mutants at normal temperatures, root elongation of fer-ts seedlings was resistant to added RALF1 peptide at elevated temperatures. Additionally, at elevated temperatures fer-ts seedlings displayed altered reactive oxygen species (ROS) accumulation upon auxin treatment and phenocopied constitutive fer mutant responses to a variety of plant hormone treatments. Molecular modeling and sequence comparison with other Catharanthus roseus receptor-like kinase 1L (CrRLK1L) receptor family members revealed that the mutated glycine in fer-ts is highly conserved, but is not located within the recently characterized RALF23 and LORELI-LIKE-GLYCOPROTEIN 2 binding domains, perhaps suggesting that fer-ts phenotypes may not be directly due to loss of binding to RALF1 peptides.