Overexpression of Sox3 is Associated with Diminished Prognosis in Esophageal Squamous Cell Carcinoma
Overexpression of Sox3 is Associated with Diminished Prognosis in Esophageal Squamous Cell Carcinoma
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Sox3 的过度表达与食管鳞状细胞癌的预后降低相关
DOI：
10.1245/s10434-012-2792-6
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发表时间：
2013-12-01
影响因子：
3.7
通讯作者：
Guo, Wei
中科院分区：
文献类型：
--
作者：
Li, Kun;Wang, Ru-Wen;Guo, Wei
背景食管鳞状细胞癌（ESCC）是一种缺乏有效预后标志物的致死性恶性肿瘤. Sox 3是高迁移率族DNA结合蛋白中的一员，可诱导鸡胚成纤维细胞发生致癌性转化。然而，Sox 3在ESCC中的表达和预后价值仍不清楚。MethodsA共30对ESCC与相应的非肿瘤性食管上皮（NE）标本的Sox 3的表达进行了研究，采用RT-PCR和Western blot分析。采用免疫组化染色法检测了包含118例ESCC和30例NE样本的组织微阵列中Sox 3的表达。结果Sox 3在食管鳞癌组织中的表达在mRNA水平和蛋白水平分别是NE的3.1倍和2.7倍（P< 0.001和P< 0.001）; Sox 3蛋白在食管鳞癌中的阳性表达率为77.1%，在NE中的阳性表达率为16.7%（P< 0.001）。Sox 3高表达与区域淋巴结转移（RLNM）（P= 0.022）和TNM分期（P= 0.011）密切相关。此外，Sox 3高表达与ESCC患者的总生存率（P< 0.001）和无复发生存率（P< 0.001）显著相关。结论Sox 3蛋白表达与食管鳞癌的发生发展有关，可作为食管鳞癌预后不良的独立预测因子。
BackgroundEsophageal squamous cell carcinoma (ESCC) is a lethal malignancy lacking valid prognostic biomarkers. As a member of the High Mobility Group domain-containing DNA-binding proteins, Sox3 has been reported to induce oncogenic transformation of chicken embryo fibroblasts. However, the expression and prognostic value of Sox3 in ESCC remain unclear.MethodsA total of 30 pairs of ESCC with a corresponding non-neoplastic esophageal epithelium (NE) specimen were investigated for Sox3 expression using RT-PCR and western blot analysis. Tissue microarrays containing 118 ESCC and 30 NE samples were detected for Sox3 expression using immunohistochemical staining. The relationship of Sox3 staining with various clinicopathological characteristics and survival of patients was statistically analyzed.ResultsSox3 expression in ESCC was 3.1- and 2.7-fold higher than in NE at mRNA (P< 0.001) and protein level (P< 0.001), respectively. Positive staining of Sox3 was observed in 77.1 % of the ESCC and 16.7 % of the NE samples (P< 0.001). High expression of Sox3 was significantly correlated with the regional lymph nodes metastasis (RLNM) (P= 0.022) and advanced TNM stage (P= 0.011). Moreover, high expression of Sox3 was significantly associated with poor overall survival (P< 0.001) and recurrence-free survival (P< 0.001) in ESCC patients. Both Sox3 expression (P< 0.001) and RLNM (P= 0.002) were independent prognostic factors for patients with ESCC.ConclusionsSox3 might play a positive role in tumor development and could serve as an independent predictor of poor prognosis for ESCC.