Proteomic analysis reveals the mechanism of different environmental stress-induced tolerance of Pseudomonas aeruginosa to monochloramine disinfection.
Proteomic analysis reveals the mechanism of different environmental stress-induced tolerance of Pseudomonas aeruginosa to monochloramine disinfection.
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DOI：
10.1016/j.jhazmat.2021.126082
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发表时间：
2021-05
影响因子：
13.6
通讯作者：
Siping Zhang;Han-Zhong Feng;Qian Wang-;S. Quan;Xiao-Quan Yu;X. Tao;Yong Wang;Ding-Ding Guo-Ding;Liang Peng;Hu-Yuan Feng;Yongxing He
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文献类型：
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作者：
Siping Zhang;Han-Zhong Feng;Qian Wang-;S. Quan;Xiao-Quan Yu;X. Tao;Yong Wang;Ding-Ding Guo-Ding;Liang Peng;Hu-Yuan Feng;Yongxing He
尽管饮用水消毒被证明是消除许多病原体的有效策略，但在饮用水分配系统中，细菌仍然可以显示出消毒耐受性。目前，环境胁迫影响铜绿假单胞菌对一氯胺耐受性的分子机制尚不清楚。在这里，我们调查了三种应激条件，即饥饿，低温，饥饿与低温相结合，如何影响铜绿假单胞菌，一种常见于饮用水分配系统中的条件致病菌的一氯胺耐受性。所有胁迫条件均能显著提高一氯胺的耐受性，其中饥饿的影响最为显著。蛋白质组学分析表明，这三种条件不仅引发了积极的抗氧化防御氧化损伤，但也准备细菌采用被动防御机制，通过休眠消毒剂。此外，抗氧化酶的表达量在饥饿条件下达到最大，进一步低温处理对细菌对氧化胁迫的响应影响不大。相反，我们发现，进一步处理饥饿的细胞与低温降低渗透胁迫反应和严格的反应，这通常发挥关键作用的消毒耐受性。综上所述，这些发现揭示了非生物因素如何影响细菌的消毒耐受性，并将有助于设计有效的策略，以消除饮用水中的铜绿假单胞菌。
Although drinking water disinfection proved to be an effective strategy to eliminate many pathogens, bacteria can still show disinfection tolerance in drinking water distribution systems. To date, the molecular mechanisms on how environmental stress affects the tolerance ofPseudomonas aeruginosato monochloramine are not well understood. Here, we investigated how three stress conditions, namely starvation, low temperature, and starvation combined with low temperature, affected the monochloramine tolerance ofPseudomonas aeruginosa, an opportunistic pathogen commonly found in drinking water distribution systems. All stress conditions significantly promoted monochloramine tolerance, among which starvation had the most drastic effects. Proteomic analyses suggested that the three conditions not only triggered a positive antioxidant defense against oxidative damages but also prepared the bacteria to employ a passive defense mechanism against disinfectants via dormancy. Moreover, the expression of antioxidant enzymes reached the maximum under the starvation condition and further low temperature treatment had little effect on bacterial response to oxidative stress. Instead, we found further treatment of the starved cells with low temperature decreased the osmotic stress response and the stringent response, which generally play pivotal roles in disinfection tolerance. Taken together, these findings shed light on how abiotic factors influence the bacterial disinfection tolerance and will aid design of efficient strategies to eliminatePseudomonas aeruginosafrom drinking water.