The biological response to nanometre-sized polymer particles.
The biological response to nanometre-sized polymer particles.
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DOI：
10.1016/j.actbio.2015.05.016
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发表时间：
2015-09
影响因子：
9.7
通讯作者：
Tipper JL
中科院分区：
文献类型：
--
作者：
Liu A;Richards L;Bladen CL;Ingham E;Fisher J;Tipper JL
最近，髋关节和膝关节置换术产生的纳米级 UHMWPE 颗粒已在体外和体内得到鉴定。 0.1–1.0 μm 尺寸范围内的 UHMWPE 颗粒已被证明比较大颗粒更具生物活性，会引发与全关节置换术后无菌性松动有关的炎症反应。此前尚未研究过纳米颗粒的生物活性。对临床相关 UHMWPE 磨损颗粒（包括纳米尺寸和微米尺寸）以及聚苯乙烯颗粒（FluoSpheres 20 nm、60 nm、200 nm 和 1.0 μm）和纳米尺寸模型聚乙烯颗粒 (Ceridust 3615®) 的生物反应是根据原代人外周血单核细胞 (PBMC) 释放的溶骨细胞因子来确定的。 12 和 24 小时后，纳米尺寸的 UHMWPE 磨损颗粒、纳米尺寸的 Ceridust 3615® 和 20 nm FluoSpheres 对 PBMNC 中 TNF-α、IL-1β、IL-6 和 IL-8 的释放没有显着影响（每个细胞浓度为 100 μm3 颗粒）。微米级 UHMWPE 磨损颗粒 (0.1–1.0 µm) 和 60 nm、200 nm 和 1.0 µm FluoSphere 导致 PBMNC 释放的溶骨细胞因子显着增加。这些结果表明，约 50 nm 以下的颗粒无法激活 PBMNC，并且颗粒大小、组成和形态在颗粒刺激的巨噬细胞释放细胞因子中发挥着至关重要的作用。
Recently, nanometre-sized UHMWPE particles generated from hip and knee replacements have been identified in vitro and in vivo. UHMWPE particles in the 0.1–1.0 μm size range have been shown to be more biologically active than larger particles, provoking an inflammatory response implicated in late aseptic loosening of total joint replacements. The biological activity of nanometre-sized particles has not previously been studied. The biological response to clinically-relevant UHMWPE wear particles including nanometre-sized and micrometre-sized, along with polystyrene particles (FluoSpheres 20 nm, 60 nm, 200 nm and 1.0 μm), and nanometre-sized model polyethylene particles (Ceridust 3615®), was determined in terms of osteolytic cytokine release from primary human peripheral blood mononuclear cells (PBMNCs). Nanometre-sized UHMWPE wear particles, nanometre-sized Ceridust 3615® and 20 nm FluoSpheres had no significant effect on TNF-α, IL-1β, IL-6 and IL-8 release from PBMNCs at a concentration of 100 μm3 particles per cell after 12 and 24 h. The micrometre-size UHMWPE wear particles (0.1–1.0 μm) and 60 nm, 200 nm and 1.0 μm FluoSpheres caused significantly elevated osteolytic cytokine release from PBMNCs. These results indicated that particles below circa 50 nm fail to activate PBMNCs and that particle size, composition and morphology played a crucial role in cytokine release by particle stimulated macrophages.