Are there multiple circadian clocks in plants?
Are there multiple circadian clocks in plants?
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DOI：
10.4161/psb.3.5.5352
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发表时间：
2008-01-01
影响因子：
2.9
通讯作者：
Webb, Alex A. R.
中科院分区：
文献类型：
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作者：
Hotta, Carlos T.;Xu, Xiaodong;Webb, Alex A. R.
我们已经报道，拟南芥可能有遗传不同的昼夜节律振荡器在多种细胞类型。1叶绿素A/B结合蛋白2（CAB 2）启动子活性的节律在光敏色素B突变体中在恒定红光下比野生型长2.5 h，在隐花色素1 cry 2双突变体（hy 4 -1 fha-1）中在恒定蓝光下比野生型长2.5 h。2然而，我们发现，在相同的光照条件下，这些突变体的胞质游离Ca ~（2+）（[Ca ~（2+）]（cyt））振荡是检测不到的。1.此外，CCA 1突变体的叶片运动具有短周期节律，但具有细胞内[Ca ~（2+）]（cyt）振荡。更重要的是，cab 1 -1（toc 1 -1）突变体的时间具有短周期的CAB 2启动子活性节律（类似于21小时），但令人惊讶的是，具有野生型的昼夜[Ca 2 +]细胞振荡周期（类似于24小时）。相比之下，TOC 1功能丧失突变体toc 1 -2具有短时间的CAB 2和[Ca 2 +] cyt节律（类似于21 h）。在这里，我们讨论toc 1 -1和toc 1 -2的表型之间的差异，以及如何节奏的CAB 2启动子活性和昼夜[Ca 2 +]（细胞色素）振荡可能会受到不同的调节。
We have reported that Arabidopsis might have genetically distinct circadian oscillators in multiple cell-types. 1 Rhythms of CHLOROPHYLL A/B BINDING PROTEIN2 (CAB2) promoter activity are 2.5 h longer in phytochromeB mutants in constant red light and in cryptocrome1 cry2 double mutant (hy4-1 fha-1) in constant blue light than the wild-type. 2 However, we found that cytosolic free Ca2+ ([Ca2+](cyt)) oscillations were undetectable in these mutants in the same light conditions. 1 Furthermore, mutants of CIRCADIAN CLOCK ASSOCIATED1 (CCA1) have short period rhythms of leaf movement but have arrhythmic [Ca2+](cyt) oscillations. More important, the timing of cab1-1 (toc1-1) mutant has short period rhythms of CAB2 promoter activity (similar to 21 h) but, surprisingly, has a wild-type period for circadian [Ca2+] cyt oscillations (similar to 24 h). In contrast, toc1-2, a TOC1 loss-of-function mutant, has a short period of both CAB2 and [Ca2+] cyt rhythms (similar to 21 h). Here we discuss the difference between the phenotypes of toc1-1 and toc1-2 and how rhythms of CAB2 promoter activity and circadian [Ca2+](cyt) oscillations might be regulated differently.