Expression profiles of insulin-like growth factor binding protein-like 1 in the developing mouse forebrain
Expression profiles of insulin-like growth factor binding protein-like 1 in the developing mouse forebrain
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DOI：
10.1016/j.modgep.2006.11.005
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发表时间：
2007-02-01
影响因子：
1.2
通讯作者：
Nakajima, Kazunori
中科院分区：
文献类型：
--
作者：
Gonda, Yuko;Sakurai, Hitoshi;Nakajima, Kazunori
胰岛素样生长因子结合蛋白样1（IGFBPL-1）具有类似于胰岛素样生长因子结合蛋白（IGFBP）的结构域结构，其是已知被胰岛素样生长因子（IGF）以高亲和力结合并由此影响细胞生长和代谢的结构域。然而，IGFBPL-1在大脑中的分布和功能尚不清楚。因此，为了阐明IGFBPL-1在发育过程中的表达谱，我们已经研究了IGFBPL-1 mRNA的表达模式，在发育中的小鼠大脑使用原位杂交，特别是前脑。在胚胎第12天，IGFBPL-1 mRNA表达定位于新皮层前板、背侧丘脑、上丘脑分化区和海马。在E14，在新皮层的室管膜下区（SVZ）和中间区（IMZ）、海马、上丘脑的分化区和背侧丘脑中识别出强表达。E14表达在所有检查的阶段中通常是最强的，此后信号降低。在E16和E18，IGFBPL-1 mRNA在海马和新皮层的SVZ中强烈检测到。在出生后的阶段，mRNA被发现只在颗粒细胞层的齿状回（DG）。值得注意的是，在P20，表达仅限于颗粒细胞层的深部，晚出生的DG神经元的位置。(c)2006 Elsevier B. V.保留所有权利。
Insulin-like growth factor binding protein-like 1 (IGFBPL-1) has a domain structure that resembles one in the insulin-like growth factor binding proteins (IGFBPs), which is a domain known to be bound by insulin-like growth factors (IGFs) with high affinity and to thereby affect cell growth and metabolism. However, the distribution and functions of IGFBPL-1 in the brain are not known. Thus, to clarify IGFBPL-1 expression profiles during development, we have investigated the expression patterns of IGFBPL-1 mRNA in developing mouse brains using in situ hybridization, focusing especially on the forebrain. On embryonic day (E)12, IGFBPL-1mRNA expressions were localized in the preplate of the neopallium, dorsal thalamus, the differentiating field of the epithalamus, and the hippocampus. On E14, strong expression was recognized in the subventricular zone (SVZ) and intermediate zone (IMZ)in the neopallium, hippocampus, the differentiating field of the epithalamus, and the dorsal thalamus. E14 expressions were generally the strongest among all the stages examined and signals decreased thereafter. At E16 and E18, IGFBPL-1mRNA was strongly detected in the hippocampus and the SVZ of the neopallium. In postnatal stages, mRNA was found only in the granule cell layer of the dentate gyrus (DG). Notably, at P20, expression was restricted to the deep fraction of the granule cell layer, the location of late-born DG neurons. (c) 2006 Elsevier B.V. All rights reserved.