Genomic characterization of a novel picornavirus in Pekin ducks
Genomic characterization of a novel picornavirus in Pekin ducks
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DOI：
10.1016/j.vetmic.2014.05.002
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发表时间：
2014-08-06
影响因子：
3.3
通讯作者：
Zhang, Dabing
中科院分区：
文献类型：
--
作者：
Liao, Qinfeng;Zheng, Lisha;Zhang, Dabing
从北京鸭（Anas platyrhynchos arctica）中检测到一种新的小核糖核酸病毒（picornavirus），并进行了全序列测定。该病毒与Megriviruses最密切相关，在P1、P2、P3、多聚蛋白以及2C和3CD区域的氨基酸同源性分别为32- 68%、35- 45%、51- 57%、41- 50%和61-63%。因此，该病毒被鉴定为Megrivirus属中的另一个物种，并命名为Duck Megrivirus（DMV）。序列分析表明，DMV基因组具有Megrivirus-like组织，也表现出一些独特的功能。多聚腺苷酸化的基因组包含9700个核苷酸，是已知小核糖核酸病毒中最大的之一。一个值得注意的特征是2A区域，其具有两个不同的、功能未知的2A（2A 1和2A 2）和一个副肠孤病毒样2A 3的关联。5'端非翻译区（UTR）含有一个变异型IVB内部核糖体进入位点（IRES），该位点具有一个长螺旋III 4，其末端为结构域III顶部的20个核苷酸长的“8”形保守结构。推测的DMV样IRES结构域III的二级结构模型在鹌鹑小核糖核酸病毒、鸽子小核糖核酸病毒B和Megriviruses中也是保守的。DMV中的结构域II包含先前在A组和C组肝炎病毒/瘟病毒样IRES中鉴定的保守的内环和顶端环。此外，DMV与不同基因组区域的不同病毒密切相关。这些发现表明，重组事件涉及交换的编码和非编码区可能已经发生。从我国4省117只鸭中检出DMV 28只，检出率为23.9%，表明我国鸭群中DMV的感染率较高。(C)2014爱思唯尔有限公司版权所有。
A novel picornavirus was detected from Pekin ducks (Anas platyrhynchos domestica) and completely sequenced. The virus was most closely related to megriviruses, with amino acid identities of 32-68%, 35-45%, 51-57%, 41-50%, and 61-63% in the P1, P2, P3, polyprotein, and 2 C and 3CD regions, respectively. The virus was thus identified as an additional species in the genus Megrivirus and named Duck megrivirus (DMV). Sequence analyses indicated that the DMV genome possessed a megrivirus-like organization and also exhibited several unique features. The polyadenylated genome comprised 9700 nt, one of the largest among known picornaviruses. A notable feature was the 2A region, which had an association of two distinct, function-unknown 2As (2A1 and 2A2) and a parechovirus-like 2A3. The 5' untranslated region (UTR) contained a variant type IVB internal ribosome entry site (IRES), which possessed a long helix III4 ending with the "8"-like 20-nt-long conserved structure at the top of domain III. The secondary structure model of inferred domain III of DMV-like IRES was also conserved in quail picornavirus, pigeon picornavirus B, and megriviruses. Domain II in DMV contained the conserved internal and apical loops previously identified in groups A and C of hepacivirus/pestivirus like IRESs. Moreover, DMV was closely related to different megriviruses in different genomic regions. These findings suggest that recombination events involving exchange of coding and noncoding regions may have occurred. DMV was detected in 28 of 117(23.9%) ducks from four provinces in China, suggesting a high prevalence of DMV in duck populations. (C) 2014 Elsevier B.V. All rights reserved.