Aqueous trifluorethanol solutions simulate the environment of DNA in the crystalline state
Aqueous trifluorethanol solutions simulate the environment of DNA in the crystalline state
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DOI：
10.1093/nar/27.17.3466
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发表时间：
1999-09-01
影响因子：
14.9
通讯作者：
Vorlícková, M
中科院分区：
文献类型：
--
作者：
Kypr, J;Chládková, J;Vorlícková, M
我们取了28个已知晶体结构的DNA片段，并使用CD光谱来寻找在溶液中稳定晶体结构的条件，所有28个片段在60-80%的三氟乙醇（TFE）水溶液中转变成它们的晶体结构，以表明晶体像浓缩的TFE一样影响DNA的构象。在B型结晶的片段也经历了合作TFE诱导的变化，发生在广泛的家庭的B型结构，这表明水和晶体B型不同，以及。精胺和镁或钙阳离子，这是包含在结晶缓冲液中，促进或抑制TFE诱导的几个片段的变化，以表明结晶剂可以决定哪些可能的结构是通过晶体中的DNA片段。
We took 28 fragments of DNA whose crystal structures were known and used CD spectroscopy to search for conditions stabilising the crystal structures in solution, All 28 fragments switched into their crystal structures in 60-80% aqueous trifluorethanol (TFE) to indicate that the crystals affected the conformation of DNA like the concentrated TFE. The fragments crystallising in the B-form also underwent cooperative TFE-induced changes that took place within the wide family of B-form structures, suggesting that the aqueous and crystal B-forms differed as well. Spermine and magnesium or calcium cations, which were contained in the crystallisation buffers, promoted or suppressed the TFE-induced changes of several fragments to indicate that the crystallisation agents can decide which of the possible structures is adopted by the DNA fragment in the crystal.