Effect of age on electrical field stimulation (EFS)-induced endothelium-dependent vasodilation in male and female rats.
Effect of age on electrical field stimulation (EFS)-induced endothelium-dependent vasodilation in male and female rats.
复制标题
年龄对雄性和雌性大鼠电场刺激（EFS）诱导的内皮依赖性血管舒张的影响。
DOI：
10.1016/s0008-6363(01)00193-6
复制
发表时间：
2001
影响因子：
10.8
通讯作者：
Davison,CA
中科院分区：
文献类型：
--
作者：
Sullivan,JC;Davison,CA
目的：本研究的目的是确定年龄对电场刺激（EFS）诱导的内皮依赖性血管舒张的影响在雄性和雌性中是否不同。方法：对年轻（3个月）和老年（25个月）Fisher 344只大鼠进行研究：年轻雌性（YF，n = 34），年轻雄性（YM，n = 28），老年雌性（OF，n = 19）和老年雄性（OM，n = 24）。对孤立的肠系膜阻力动脉（内皮完整和剥脱）进行加压，并监测外径。 EFS 反应曲线（0.1–8 Hz）是在存在胍乙啶的情况下在预收缩动脉中进行的。 EFS 反应表示为预收缩直径的松弛百分比。计算曲线下面积（AUC）并使用方差分析和t检验进行比较。结果：随着年龄的增长，男性对EFS诱导的血管舒张的反应逐渐减弱，而女性的反应不受影响（AUC：YM=344±23，OM=253±25，P=0.008；YF=397±21，OF=365±25，P=0.33）。在所有大鼠组中，内皮剥脱导致 EFS 诱导的扩张显着减少。与年老动物相比，剥蚀对年轻动物的影响更大。与一氧化氮合酶抑制剂 Nω-硝基-L-精氨酸 (LNA) 一起孵育可显着降低所有大鼠组的 EFS 反应性。结论：EFS 诱导的血管舒张功能随着雄性大鼠年龄的增长而减弱。在 YF 和 YM 中，内皮依赖性 EFS 诱导的血管舒张是由内皮衍生的 NO 的释放介导的。随着年龄的增长，内皮功能下降，这可能是由于内皮源性 NO 的产生减少所致。内皮源性 NO 的减少可能是男性 EFS 诱导的血管舒张随着年龄增长而减少的原因。
Objective:The purpose of this study was to determine whether the effect of age on electrical field stimulation (EFS)-induced endothelium-dependent vasodilation was different in males and females.Methods:Young (3 month) and old (25 month) Fisher 344 rats were studied: young females (YF,n= 34), young males (YM,n= 28), old females (OF,n= 19), and old males (OM,n= 24). Isolated mesenteric resistance arteries (endothelium-intact and denuded) were pressurized, and outer diameter was monitored. EFS-response curves (0.1–8 Hz) were performed in preconstricted arteries in the presence of guanethidine. EFS responses were expressed as percent relaxation from preconstricted diameter. Area under the curve (AUC) was calculated and comparisons were made using ANOVA andt-tests.Results:Males became less responsive to EFS-induced vasodilation with age, while responses among females were unaffected (AUC: YM=344±23, OM=253±25,P= 0.008; YF=397±21, OF=365±25,P= 0.33). Endothelial denudation produced a significant decrease in EFS-induced dilation among all rat groups. The effect of denudation was greater in young animals compared to old. Incubation with the nitric oxide synthase inhibitorNω-nitro-l-arginine (LNA) significantly decreased EFS responsiveness among all of the rat groups.Conclusions:EFS-induced vasodilation declines with age among males. In YF and YM endothelium-dependent EFS-induced vasodilation is mediated by the release of endothelium-derived NO. With age, endothelial function declines, which is likely due to a decrease in the production of endothelium-derived NO. A decline in endothelial-derived NO is likely responsible for the decrease in EFS-induced vasodilation with age among males.