Tail morphology controls DNA release in two Salmonella phages with one lipopolysaccharide receptor recognition system
Tail morphology controls DNA release in two Salmonella phages with one lipopolysaccharide receptor recognition system
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DOI：
10.1111/j.1365-2958.2012.08006.x
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发表时间：
2012-03-01
影响因子：
3.6
通讯作者：
Barbirz, Stefanie
中科院分区：
文献类型：
--
作者：
Andres, Dorothee;Roske, Yvette;Barbirz, Stefanie
噬菌体利用特定的尾部蛋白质来识别宿主细胞。信号如何通过尾部传递以启动感染，其分子细节仍不清楚。我们分析了长尾丝状噬菌体9NA和短尾短尾噬菌体P22在与鼠伤寒沙门氏菌脂多糖（LPS）孵育时的体外DNA释放情况。我们首次表明，仅LPS就足以在体外引发丝状噬菌体的DNA释放。晶体结构分析显示，两种噬菌体都使用相似的尾刺蛋白来识别LPS。尾刺蛋白水解LPS的O抗原，使噬菌体定位在细胞表面。因此，我们能够在相同的实验条件下，比较两种形态不同但具有相同受体的噬菌体的体外DNA释放过程。丝状噬菌体9NA释放其DNA的速度比短尾噬菌体P22快约30倍。DNA释放受粒子构象开放的控制，并且在9NA和P22中具有相似的激活屏障。我们的数据表明，在给定相同的初始受体相互作用事件的情况下，尾部形态会影响粒子开放的效率。
Bacteriophages use specific tail proteins to recognize host cells. It is still not understood to molecular detail how the signal is transmitted over the tail to initiate infection. We have analysed in vitro DNA ejection in long-tailed siphovirus 9NA and short-tailed podovirus P22 upon incubation with Salmonella typhimurium lipopolysaccharide (LPS). We showed for the first time that LPS alone was sufficient to elicit DNA release from a siphovirus in vitro. Crystal structure analysis revealed that both phages use similar tailspike proteins for LPS recognition. Tailspike proteins hydrolyse LPS O antigen to position the phage on the cell surface. Thus we were able to compare in vitro DNA ejection processes from two phages with different morphologies with the same receptor under identical experimental conditions. Siphovirus 9NA ejected its DNA about 30 times faster than podovirus P22. DNA ejection is under control of the conformational opening of the particle and has a similar activation barrier in 9NA and P22. Our data suggest that tail morphology influences the efficiencies of particle opening given an identical initial receptor interaction event.