Construction of a multifunctional nanoprobe for tumor-targeted time-gated luminescence and magnetic resonance imaging in vitro and in vivo
Construction of a multifunctional nanoprobe for tumor-targeted time-gated luminescence and magnetic resonance imaging in vitro and in vivo
复制标题
构建用于体外和体内肿瘤靶向时间选通发光和磁共振成像的多功能纳米探针
DOI：
10.1039/c8nr03085e
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发表时间：
2018
期刊：
影响因子：
6.7
通讯作者：
Yuan Jingli
中科院分区：
文献类型：
--
作者：
Dai Zhichao;Ma Hua;Tian Lu;Song Bo;Tan Mingqian;Zheng Xiuwen;Yuan Jingli
双模态荧光-磁共振成像技术因其在早期肿瘤诊断的高准确性方面的潜力而受到极大的关注。在这项研究中，一个简单的方法已经开发到制备肿瘤靶向纳米探针，PTTA-Eu 3 +-CoFeO-FA纳米粒子，用于双模式时间门控发光（TGL）-磁共振（MR）成像的肿瘤细胞在体外和体内。通过将肿瘤靶向分子叶酸（FA）和发光Eu 3+络合物PTTA-Eu 3+涂覆到钴/氧化铁（CoFeO）纳米颗粒的表面上来构建多功能纳米探针。所制备的PTTA-Eu 3 +-CoFeO-FA纳米粒子在水中分散良好，具有良好的生物相容性、较强的长寿命发光以及显著的横向弛豫。体外研究表明，该纳米探针可作为一种有效的发光探针，实现RAW 264. 7细胞的靶向TGL成像，且不受背景荧光的干扰;体内双模式TGL-MR成像结果表明，所制备的纳米探针可优先在肿瘤中聚集，有效增强T2加权MR成像和TGL成像的信号。研究成果将有助于开发新型双模荧光-MR纳米探针，用于肿瘤的临床诊断和治疗。
A dual-modal fluorescence-magnetic resonance imaging technique has gained tremendous attention for its potential in the dawning era of early diagnosis of tumors with high accuracy. In this study, a facile approach has been developed to prepare a tumor-targetable nanoprobe, PTTA-Eu3+-CoFeO-FA nanoparticles, for dual-modal time-gated luminescence (TGL)–magnetic resonance (MR) imaging of tumor cells in vitro and in vivo. The multifunctional nanoprobe was constructed by coating a tumor-targeting molecule, folic acid (FA), and a luminescent Eu3+ complex, PTTA-Eu3+, onto the surface of cobalt/iron oxide (CoFeO) nanoparticles. The as-prepared PTTA-Eu3+-CoFeO-FA nanoparticles are well dispersed in water with good biocompatibility, strong long-lived luminescence as well as pronounced transverse relaxivity. The in vitro study reveals that the nanoprobe works well as an effective luminescent probe to achieve the targeted TGL imaging of RAW 264.7 cells without the interference of background fluorescence, and the results of in vivo dual-modal TGL–MR imaging indicate that the fabricated nanoprobe can be preferentially accumulated in the tumor to effectively enhance the signals of T2-weighted MR imaging and TGL imaging. The research achievements will contribute to the development of new dual-modal fluorescence-MR nanoprobes for application in clinical diagnosis and therapy of tumors.