Aortic adventitial angiogenesis and lymphangiogenesis promote intimal inflammation and hyperplasia
Aortic adventitial angiogenesis and lymphangiogenesis promote intimal inflammation and hyperplasia
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主动脉外膜血管生成和淋巴管生成促进内膜炎症和增生
DOI：
10.1016/j.carpath.2008.07.004
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发表时间：
2009-09-01
影响因子：
3.7
通讯作者：
Zhang, Yun
中科院分区：
文献类型：
--
作者：
Xu, Xinsheng;Lu, Huixia;Zhang, Yun
简介：众所周知，膜外炎症会影响新内膜的形成和血管重塑。本研究旨在阐明球囊诱导的主动脉内皮损伤后新生内膜增生与外膜血管生成和淋巴管生成的关系。方法：70只雄性Wistar大鼠随机分为6个干预组和1个对照组。测定大鼠主动脉内膜面积/内侧面积比（I/M ratio）、内皮巨噬细胞指数、内皮微血管（Ad-MV）和淋巴管（Ad-LV）数量，采用实时荧光定量pcr法测定主动脉壁VEGF-A、VEGFR-1、VEGF-C、VEGFR-3、PDGF-B、pdgfr - β mRNA表达。结果：与对照组比较，介入组在球囊损伤后I/M比值、巨噬细胞指数、Ad-MV、Ad-LV以及VEGF-A、VEGFR-1、VEGF-C、VEGFR-3、PDGF-B、pdgfr - β的rnRNA表达均显著升高。球囊介入后I/M比值与Ad-MV、Ad-LV呈显著相关。多元线性回归分析显示Ad-MV和Ad-LV是内膜增生的独立因素。结论：球囊损伤后内膜炎症可诱导血管新生和淋巴管新生，这些新生血管可能通过传递和激活炎症细胞促进内膜炎症和增生。(C) 2009爱思唯尔公司版权所有。
Introduction: Adventitial inflammation is known to influence neointimal formation and vascular remodeling. The present study was aimed to clarify the relationship between neointima hyperplasia and adventitial angiogenesis and lymphangiogenesis after balloon-induced aortic endothelial injury. Methods: Seventy male Wistar rats were randomly divided into six interventional groups and one control group. The intimal area/medial area ratio (I/M ratio), the adventitial macrophage index, and the number of adventitial microvessels (Ad-MV) and lymphatic vessels (Ad-LV) in the aorta were nicasured, and the mRNA expressions of VEGF-A, VEGFR-1, VEGF-C, VEGFR-3, PDGF-B, and PDGFR-beta in the aortic wall were quantified by real-time RT-PCR. Results: Compared with the control group, the I/M ratio, macrophage index, Ad-MV, Ad-LV, and the rnRNA expressions of VEGF-A, VEGFR-1, VEGF-C, VEGFR-3, PDGF-B, and PDGFR-beta in interventional groups increased significantly after balloon-induced injury. I/M ratio showed significant correlations with Ad-MV and Ad-LV after balloon intervention. Multiple linear regression analysis indicated that Ad-MV and Ad-LV were independent factors of intimal hyperplasia. Conclusion: Adventitial angiogenesis and lymphangiogenesis are induced by intimal inflammation after balloon injury, and these neogenetic vessels in turn promote intimal inflammation and hyperplasia probably via delivery and activation of inflammatory cells. (C) 2009 Elsevier Inc. All rights reserved.