Reactive astrocytes contribute to increased epileptic susceptibility induced by subthreshold dose of pilocarpine
Reactive astrocytes contribute to increased epileptic susceptibility induced by subthreshold dose of pilocarpine
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反应性星形胶质细胞导致阈下剂量的毛果芸香碱诱导的癫痫易感性增加
DOI：
10.1016/j.yebeh.2012.08.023
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发表时间：
2012-11-01
影响因子：
2.6
通讯作者：
Pan, Xiao-Hong
中科院分区：
文献类型：
--
作者：
Zhu, Wei;Zhang, Shi-Hong;Pan, Xiao-Hong
癫痫发作可能影响癫痫发生，但尚不清楚阈下刺激是否足以诱导可见的行为性癫痫发作改变癫痫易感性，并且可能的潜在机制尚未完全了解。我们用免疫组化方法评估了阈下剂量匹罗卡品对癫痫的易感性，以及神经胶质纤维酸性蛋白（GFAP）的表达。对戊四唑（PTZ）诱发的癫痫发作的易感性增加在先前接受阈下剂量匹罗卡品的大鼠中观察到。GFAP的免疫反应性也增强，表明星形胶质细胞在某些脑亚区发生反应。星形细胞功能抑制剂l - α -氨基己二酸（L-AAA）可显著降低癫痫易感性。我们的研究结果表明，阈下刺激可能会增加癫痫后续发展的易感性，而反应性星形胶质细胞可能是这一过程的重要因素。充分抑制星形细胞功能可能是预防癫痫发生的一种潜在方法。(C) 2012爱思唯尔公司版权所有。
Seizures may influence epileptogenesis, but it is not yet clearly established whether subthreshold stimulations that are not sufficient to induce visible behavioral seizures change epileptic susceptibility, and the possible underlying mechanisms have not been completely understood. We assessed the susceptibility to epilepsy after subthreshold dose of pilocarpine, as well as glial fibrillary acidic protein (GFAP) expression using immunohistochemistry. An increase in the susceptibility to pentylenetetrazole (PTZ)-induced seizures was observed in rats previously subjected to subthreshold dose of pilocarpine. The immunoreactivity of GFAP was also increased, indicating that astrocytes became reactive in some brain subfields. The increased epileptic susceptibility was significantly reduced by L-alpha-aminoadipic acid (L-AAA), an inhibitor of astrocytic function. Our results suggest that subthreshold stimulation may increase the susceptibility to subsequent development of epilepsy, and reactive astrocytes might be an important contributor to this process. Adequate inhibition of astrocytic function may be a potential preventive approach against epileptogenesis. (C) 2012 Elsevier Inc. All rights reserved.