Differential expression of two soybean apyrases, one of which is an early nodulin
Differential expression of two soybean apyrases, one of which is an early nodulin
复制标题
DOI：
10.1094/mpmi.2000.13.10.1053
复制
发表时间：
2000-10-01
影响因子：
3.5
通讯作者：
Stacey, G
中科院分区：
文献类型：
--
作者：
Day, RB;McAlvin, CB;Stacey, G
采用聚合酶链反应从大豆（甘氨酸大豆）中分离出两个cDNA克隆，引物设计为核酸水解酶（核苷酸磷酸水解酶）中的保守基序。预计这两种cdna编码两种不同的apyrase蛋白，大约50 kDa（即GS50）和52 kDa（即GS52）。系统发育分析表明，GS52与最近被认为在豆科植物结瘤中起作用的一个apyrase家族同源。GS50与该家族相似，因此可能发挥不同的生理作用。与此分析一致，GS50 mRNA在根、下胚轴、花和茎中检测到，GS52 mRNA在根和花中检测到，在叶和子叶中均未表达。用大豆的固氮共生体——缓生根瘤菌日本根瘤菌接种根瘤菌，可使大豆快速生长。
Two cDNA clones were isolated from soybean (Glycine soja) by polymerase chain reaction with primers designed to conserved motifs found in apyrases (nucleotide phosphohydrolase). The two cDNAs are predicted to encode for two, distinct, apyrase proteins of approximately 50 kDa (i.e., GS50) and 52 kDa (i.e,, GS52). Phylogenetic analysis indicated that GS52 is orthologous to a family of apyrases recently suggested to play a role in legume nodulation. GS50 is paralogous to this family and, therefore, likely plays a different physiological role. Consistent with this analysis, GS50 mRNA was detected in root, hypocotyls, flowers, and stems, while GS52 mRNA was found in root and flowers, Neither gene was expressed in leaves or cotyledons. Inoculation of roots with Bradyrhizobium japonicum, nitrogen-fixing symbiont of soybean, resulted in the rapid (