VEGF and flt - Expression time kinetics in rat brain infarct
VEGF and flt - Expression time kinetics in rat brain infarct
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DOI：
10.1161/01.str.27.10.1865
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发表时间：
1996-10-01
期刊：
影响因子：
8.3
通讯作者：
Fukui, M
中科院分区：
文献类型：
--
作者：
Kovacs, Z;Ikezaki, K;Fukui, M
背景和目的血管内皮生长-血管通透性因子（VEGF）是一种可分泌的内皮细胞特异性有丝分裂原，在缺氧时表达上调，是一种血管生成和高通透性诱导因子。本研究旨在阐明VEGF及其受体系统在实验性脑梗死中的时序性表达。采用免疫组织化学方法，半定量分析VEGF/flt系统在脑梗死后3 h ~ 3周不同时间段的表达。在38和45 kD免疫印迹上获得的条带与VEGF（121）和VEGF（165）亚型的条带相关。巨噬细胞、神经元和胶质细胞按时间顺序以不同的方式表达VEGF免疫反应性。VEGF（结合）和fit检测到内皮细胞沿着血管生成的发展。结论在缺血性脑巨噬细胞，神经元和胶质细胞似乎含有VEGF。血管内皮生长因子受体的拟合沿着梗死血管生成的进展。因此认为VEGF/flt系统参与脑梗死的愈合过程。
Background and Purpose Vascular endothelial growth-vascular permeability factor (VEGF) is a candidate for an angiogenic and hyperpermeability inducing factor in an infarct because it is a secretable mitogen specific for endothelial cells and is upregulated by hypoxia. Our study attempts to clarify the chronological expression of VEGF and its receptor (fit) system in experimental cerebral infarction.Methods With the use of a reproducible middle cerebral artery occlusion model in rats, VEGF expression was identified by Western blotting with anti-VEGF antibody. The chronological expression of the VEGF/flt system was analyzed semiquantitatively by immunohistochemical means in infarcts with different time courses from 3 hours to 3 weeks.Results VEGF and fit were expressed exclusively in the ischemic brain. The bands obtained on the immunoblot at 38 and 45 kD are related td those of VEGF(121) and VEGF(165) isoforms. Macrophages, neurons, and glial cells chronologically expressed VEGF immunoreactivity in a different fashion. Both VEGF (bound) and fit were detected in endothelial cells along with the development of angiogenesis.Conclusions In the ischemic brain the macrophages, neurons, and glial cells appear to contain VEGF. The VEGF receptor fit was induced in endothelial cells along with the progression of angiogenesis in infarct. The VEGF/flt system is thus considered to be involved in the healing process of brain infarct.