Remote Loading of Small-Molecule Therapeutics into Cholesterol-Enriched Cell-Membrane-Derived Vesicles.
Remote Loading of Small-Molecule Therapeutics into Cholesterol-Enriched Cell-Membrane-Derived Vesicles.
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DOI：
10.1002/anie.201707598
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发表时间：
2017-11-06
期刊：
影响因子：
--
通讯作者：
Zhang L
中科院分区：
文献类型：
--
作者：
Zhang X;Angsantikul P;Ying M;Zhuang J;Zhang Q;Wei X;Jiang Y;Zhang Y;Dehaini D;Chen M;Chen Y;Gao W;Fang RH;Zhang L
仿生设计原理在纳米医学中的日益流行导致了性能和生物相容性增强的治疗平台的出现。这包括使用天然衍生的细胞膜，它可以赋予纳米载体细胞特有的功能。在这里，我们报告了一种策略，通过远程加载到来自红细胞的膜微囊中，能够有效地包裹药物。这是通过在膜上添加额外的胆固醇、稳定纳米结构和促进pH梯度的保留来实现的。我们展示了两种模型药物的负载：化疗药物阿霉素和抗生素万古霉素。使用动物疾病模型在体外和体内研究了这些天然的、远程加载的纳米制剂的治疗意义。最终，这种方法可以用来设计新的仿生纳米制剂，具有更高的疗效和更好的安全性。远程加载的红细胞囊泡是通过在天然来源的细胞膜上添加额外的胆固醇来制造的。阿霉素和万古霉素两种模型药物已成功加载，并在疾病动物模型中显示出疗效。报道的方法可能为高效和安全的仿生纳米制剂铺平道路
The increasing popularity of biomimetic design principles in nanomedicine has led to therapeutic platforms with enhanced performance and biocompatibility. This includes the use of naturally derived cell membranes, which can bestow nanocarriers with cell-specific functionalities. Herein, we report on a strategy enabling efficient encapsulation of drugs via remote loading into membrane vesicles derived from red blood cells. This is accomplished by supplementing the membrane with additional cholesterol, stabilizing the nanostructure and facilitating the retention of a pH gradient. We demonstrate the loading of two model drugs: the chemotherapeutic doxorubicin and the antibiotic vancomycin. The therapeutic implications of these natural, remote-loaded nanoformulations are studied both in vitro and in vivo using animal disease models. Ultimately, this approach could be used to design new biomimetic nanoformulations with higher efficacy and improved safety profiles. Remotely loaded RBC vesicles are fabricated by enriching the naturally derived cell membrane with additional cholesterol. Two model drugs, doxorubicin and vancomycin, are successfully loaded and show efficacy in animal models of disease. The reported approach may pave the way for biomimetic nanoformulations with high potency and safety
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影响因子：
9.8
作者：
Collins SR;Douglass A;Vale RD;Weissman JS
通讯作者：
Weissman JS
影响因子：
2.9
作者：
Lim KH;Dasari AK;Hung I;Gan Z;Kelly JW;Wemmer DE
通讯作者：
Wemmer DE
DOI：
10.1074/mcp.m114.044610
发表时间：
2015-05
期刊：
Molecular & cellular proteomics : MCP
影响因子：
--
作者：
Scarff CA;Almeida B;Fraga J;Macedo-Ribeiro S;Radford SE;Ashcroft AE
通讯作者：
Ashcroft AE
影响因子：
3.3
作者：
Crespo, Rosa;Villar-Alvarez, Eva;Martins, Pedro M.
通讯作者：
Martins, Pedro M.
影响因子：
2.9
作者：
Noda, Sayaka;So, Masatomo;Goto, Yuji
通讯作者：
Goto, Yuji