Aggregation and dispersion characteristics of calcium oxalate monohydrate: Effect of urinary species
Aggregation and dispersion characteristics of calcium oxalate monohydrate: Effect of urinary species
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DOI：
10.1006/jcis.2002.8283
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发表时间：
2002-12-01
影响因子：
9.9
通讯作者：
El-Shall, H
中科院分区：
文献类型：
--
作者：
Christmas, KG;Gower, LB;El-Shall, H
在这项研究中，采用筛选和中心复合实验设计来确定各种因素对先前生长的一水草酸钙（COM）晶体在受控变量的人工泌尿环境中的聚集和分散特性的影响。检查的变量是超纯水和人工尿液中的 pH 值以及钙、草酸盐、焦磷酸盐、柠檬酸盐和蛋白质浓度。光密度测量、粒度分析、光学显微镜、AFM 力测量和蛋白质吸附已用于评估 COM 晶体的聚集和分散状态，并阐明此类复杂系统中涉及的机制。数据表明我们的模型蛋白粘蛋白起到分散剂的作用。这归因于吸附的粘蛋白造成的空间位阻。然而，草酸盐会促进聚集。观察到蛋白质和草酸盐以及蛋白质和柠檬酸盐之间有趣的相互作用。发现这种相互作用（协同或拮抗）取决于这些物质的浓度。本文介绍并讨论了这些相互作用的表面响应。总之，溶液、表面和界面化学物质在生理环境中以复杂的方式相互作用，抑制或促进聚集，了解这种相互作用可能有助于确定和控制影响肾结石形成的因素。 (C) 2002 年爱思唯尔科学（美国）。
In this research, screening and central composite experimental designs are used to determine the effect of various factors on the aggregation and dispersion characteristics of previously grown calcium oxalate monohydrate (COM) crystals in artificial urinary environments of controlled variables. The variables examined are pH and calcium, oxalate, pyrophosphate, citrate, and protein concentrations in ultrapure water and artificial urine. Optical density measurements, particle size analysis, optical microscopy, AFM force measurements, and protein adsorption have been used to assess the state of aggregation and dispersion of the COM crystals and to elucidate the mechanisms involved in such a complex system. The data indicate that our model protein, mucin, acts as a dispersant. This is attributed to steric hindrance resulting from the adsorbed mucoprotein. Oxalate, however, promotes aggregation. Interesting interactions between protein and oxalate along with protein and citrate are observed. Such interactions (synergistic or antagonistic) are found to depend on the concentrations of these species. Surface responses for these interactions are presented and discussed in this paper. In summary, solution, surface, and interface chemistries interact in a complex manner in the physiological environment to either inhibit or promote aggregation, and an understanding of such interactions may help determine and control the factors affecting kidney stone formation. (C) 2002 Elsevier Science (USA).