METABOLISM OF CYCLOOXYGENASE AND LIPOXYGENASE PRODUCTS BY 15-PROSTAGLANDIN DEHYDROGENASE FROM HUMAN HL-60 LEUKEMIA-CELLS
METABOLISM OF CYCLOOXYGENASE AND LIPOXYGENASE PRODUCTS BY 15-PROSTAGLANDIN DEHYDROGENASE FROM HUMAN HL-60 LEUKEMIA-CELLS
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DOI：
10.1007/bf01966527
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发表时间：
1987-08-01
期刊：
影响因子：
--
通讯作者：
TAFFER, IM
中科院分区：
文献类型：
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作者：
AGINS, AP;ZIPKIN, RE;TAFFER, IM
本文研究了几种洋地黄素和单羟化脂肪酸作为NAD依赖性15-前列腺素脱氢酶（15-PGDH）的底物，该酶从人HL-60细胞中获得。具有w-6羟基部分的脂氧合酶和环氧合酶衍生底物（15-HETE、13-HODD和HHT）代谢为相应的酮衍生物，代谢速率与野牡丹素E2和F2α相当。12-HETE是一种不良底物，5-HETE未被代谢。w-6羟基脂肪酸抑制PGE 2的代谢，因此它们可能在调节生物活性前列腺素水平中起作用。
A number of prostaglandins and mono-hyroxylated fatty acids were investigated as substrates for NAD-dependent 15-prostaglandin dehydrogenase (15-PGDH) obtained from human HL-60 cells. Lipoxygenase and cyclooxygenase-derived substrates possessing an w-6 hydroxyl moiety (15-HETE, 13-HODD and HHT) were metabolized to corresponding keto derivatives at a rate comparable to that for prostaglandins E2and F2α. 12-HETE was a poor substrate and 5-HETE was not metabolized. The w-6 hydroxy fatty acids inhibited the metabolism of PGE2and therefore they may play a role in modulating bioactive prostaglandin levels.