ESTIMATES OF INVIVO INSULIN ACTION IN MAN - COMPARISON OF INSULIN TOLERANCE-TESTS WITH EUGLYCEMIC AND HYPERGLYCEMIC GLUCOSE CLAMP STUDIES
ESTIMATES OF INVIVO INSULIN ACTION IN MAN - COMPARISON OF INSULIN TOLERANCE-TESTS WITH EUGLYCEMIC AND HYPERGLYCEMIC GLUCOSE CLAMP STUDIES
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DOI：
10.1210/jcem-68-2-374
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发表时间：
1989-02-01
影响因子：
5.8
通讯作者：
MUGGEO, M
中科院分区：
文献类型：
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作者：
BONORA, E;MOGHETTI, P;MUGGEO, M
我们比较了17名正常受试者和19名患有各种胰岛素抵抗疾病的患者的胰岛素耐量试验（ITT）和正常血糖和高血糖葡萄糖钳夹研究的体内胰岛素作用估计值。15例受试者接受了ITT和正葡萄糖钳夹研究，17例受试者接受了ITT和高葡萄糖钳夹研究，4例受试者接受了所有3项试验。ITT包括常规胰岛素静脉推注（0.1 U/kg BW）。胰岛素注射后3- 15分钟内的血糖消失率作为胰岛素作用的指标。在正常血糖钳夹和高血糖钳夹研究中，将葡萄糖输注量与60-120 min（M）（正常血糖钳夹研究）或20-120 min（I）（高血糖钳夹研究）的平均血浆胰岛素浓度之间的比值用作胰岛素作用的量度。正常血糖（r = 0.811; P < 0.001）和高血糖（r = 0.826; P <0.001）时，血糖消失率与M/I比值均呈显著正相关。这些结果表明，当葡萄糖钳夹研究不可行时，如在大系列受试者或系列研究中，15分钟ITT适合作为体内胰岛素作用的简单快速估计。
We compared estimates of in vivo insulin action derived from insulin tolerance tests (ITT) and euglycemic and hyperglycemic glucose clamp studies in 17 normal subjects and 19 patients with various diseases characterized by insulin resistance. Fifteen subjects underwent an ITT and a euglycemic clamp study, 17 subjects underwent an ITT and a hyperglycemic clamp study, and 4 subjects underwent all 3 tests. The ITT consisted of a bolus iv injection of regular insulin (0.1 U/kg BW). The plasma glucose disappearance rate during the 3- to 15-min period following the insulin injection was taken as a measure of insulin action. In both euglycemic and hyperglycemic clamp studies, the ratio between the amount of glucose infused to maintain glycemia at the desired level and the mean plasma insulin concentration from 60-120 min (M) (euglycemic clamp studies) or 20-120 min (I) (hyperglycemic clamp studies) was used as a measure of insulin action. A close correlation was found between plasma glucose disappearance rate and the M/I ratio during either the euglycemic (r = 0.811; P < 0.001) or the hyperglycemic (r = 0.826; P < 0.001) clamp sutides. These results suggest that the 15-min ITT is suitable as a simple and rapid estimation of in vivo insulin action when glucose clamp studies are not feasible, as in large series of subjects or serial studies.