Age related changes in the non-collagenous components of the extracellular matrix of the human lamina cribrosa
Age related changes in the non-collagenous components of the extracellular matrix of the human lamina cribrosa
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DOI：
10.1136/bjo.84.3.311
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发表时间：
2000-03-01
影响因子：
4.1
通讯作者：
Duance, VC
中科院分区：
文献类型：
--
作者：
Albon, J;Karwatowski, WSS;Duance, VC
目的-为了探讨年龄相关的变化，在非胶原成分的人筛板。方法-纤维连接蛋白，弹性蛋白和胶质细胞酸性蛋白（GFAP）染色进行了评估，在年轻和年老的筛板。年龄范围分别采用氯仿-甲醇提取法、荧光测定法、二甲基亚甲基蓝测定法和离子交换色谱法，分析了人筛板（7天至96岁）的脂质含量（n=9）、细胞结构（n = 28）、总硫酸化糖胺聚糖（n=28）、弹性蛋白含量（n=9）和蛋白质含量（n=56）。当年轻组织与老年组织相比时，弹性蛋白增加，纤连蛋白和GFAP减少。老化的人筛板的生化分析表明，弹性蛋白含量从8%增加到28%干组织重量，总硫酸化糖胺聚糖减少，脂质含量从45%下降到25%。有没有显着的变化，总cellularity或watercontents. Conclusion，这些变化的组合物可能是指示筛板的代谢状态，因为它的年龄，并可能有助于机械完整性的变化。这种变化可能与老年筛板的易感性及其对青光眼视神经病变的反应有关。
Aims-To investigate age related alterations in the non-collagenous components of the human lamina cribrosa.Methods-Fibronectin, elastin; and glial fibrillary acidic protein (GFAP) staining were assessed in young and old laminae cribrosae. An age range (7 days to 96 years) of human laminae cribrosae were analysed for lipid content (n=9), cellularity (n=28), total sulphated glycosaminoglycans (n=28), elastin content (n=9), and waiter content (n=56), using chloroform-methanol extraction, fluorimetry, the dimethylmethylene blue assay, and ion exchange chromatography, respectively.Results-Qualitatively, an increase in elastin and a decrease in fibronectin and GFAP were demonstrated when young tissue was compared with the elderly. Biochemical analysis of the ageing human lamina cribrosa demonstrated that elastin content increased from 8% to 28% dry tissue weight, total sulphated glycosaminoglycans decreased, and lipid content decreased from 45% to 25%. There were no significant changes in total cellularity or water content.Conclusion-These alterations in composition may be indicative of the metabolic state of the lamina cribrosa as it ages, and may contribute to changes in mechanical integrity. Such changes may be implicated in the susceptibility of the elderly lamina cribrosa and also its response to glaucomatous optic neuropathy.