Bartonella vinsonii subsp berkhoffii subsp nov, isolated from dogs; Bartonella vinsonii subsp vinsonii; And emended description of Bartonella vinsonii
Bartonella vinsonii subsp berkhoffii subsp nov, isolated from dogs; Bartonella vinsonii subsp vinsonii; And emended description of Bartonella vinsonii
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DOI：
10.1099/00207713-46-3-704
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发表时间：
1996-07-01
期刊：
影响因子：
--
通讯作者：
Brenner, DJ
中科院分区：
文献类型：
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作者：
Kordick, DL;Swaminathan, B;Brenner, DJ
两株细菌，一株从瓣膜心内膜炎犬的血液中分离出来，另一株从健康犬的血液中分离出来，经生长特性、生化反应和细胞壁脂肪酸组成等多项表型标准测定，与巴尔通体相似。16S rRNA基因序列相似性研究结果证实，这两株细菌具有密切的亲缘关系，属于巴尔通体属，文森巴尔通体是它们最近的亲缘关系(分离株93-C01(T)[T=型株]的16S rRNA与文氏巴尔通体的16S rRNA同源性为99.37%，G7464分离株的16S rRNA与模式株的16S rRNA同源性为99.61%，犬分离株16S rRNA的同源性为99.77%)，两个分离株的16S rRNA均含有巴尔通体16S rRNA中不存在的12个碱基的插入。DNA亲缘关系分析表明，这些菌株在种水平上与文森伯氏杆菌模式株有较近的亲缘关系，但它们之间的亲缘关系明显大于与文森伯氏杆菌的亲缘关系。根据它们独特的16S rRNA序列插入、较高的亲缘关系以及它们相似的起源(狗)，我们认为93-C01(T)和G7464菌株应该被归入文氏杆菌的一个亚种，我们建议将其命名为B，vinsonii亚种，伯霍夫亚种，nov，模式菌株为93-C01(=atcc 51672)。为适应这一新亚种，对文氏假单胞菌的描述进行了修订，并对文氏假单胞菌亚种进行了描述。
Two bacterial strains, one isolated from the blood of a dog with valvular endocarditis and one isolated from the blood of a healthy dog, were similar to Bartonella species, as determined by a number of phenotypic criteria, including growth characteristics, biochemical reactions, and cell wall fatty acid composition, The results of 16S rRNA gene sequence similarity studies confirmed that these strains are closely related and belong in the genus Bartonella and that Bartonella vinsonii is their closest relative (the 16S rRNA of isolate 93-C01(T) [T = type strain] was 99.37% identical to the 16S rRNA of the type strain of B. vinsonii, the 16S rRNA of isolate G7464 was 99.61% identical to the 16S rRNA of the type strain, and the 16S rRNAs of the dog isolates were 99.77% identical to each other), The 16S rRNAs of both strains contained a 12-base insertion that was not present in the 16S rRNA of the type strain of any Bartonella species. DNA relatedness tests revealed that these strains were related at the species level to the type strain of B. vinsonii, They were, however, significantly more closely related to each other than to B. vinsonii. On the basis of their unique 16S rRNA sequence insertion, their preferentially high level of relatedness, and their similar origins (dogs), we believe that strains 93-C01(T) and G7464 should be placed in a separate subspecies of B. vinsonii, for which we propose the name B, vinsonii subsp, berkhoffii subsp, nov, The type strain of B. vinsonii subsp, berkhoffii is strain 93-C01 (= ATCC 51672). The description of B. vinsonii is emended to accommodate the new subspecies, and B. vinsonii subsp, vinsonii is described.