In Vitro Evaluation of the Ciliary Beat Frequency of the Rat Nasal Epithelium Using a High-Speed Digital Imaging System
In Vitro Evaluation of the Ciliary Beat Frequency of the Rat Nasal Epithelium Using a High-Speed Digital Imaging System
复制标题
DOI：
10.1248/bpb.b12-01076
复制
发表时间：
2013-06-01
影响因子：
2
通讯作者：
Higashi, Yutaka
中科院分区：
文献类型：
--
作者：
Inoue, Daisuke;Furubayashi, Tomoyuki;Higashi, Yutaka
粘膜纤毛清除率（Mucociliary clearance， MC）是决定鼻腔药物吸收的重要因素，鼻黏膜纤毛上皮细胞的纤毛搏动是MC的驱动力，但MC与纤毛搏动频率（ciliary beat frequency， CBF）之间的关系尚不明确。本研究旨在建立一种评价纤毛粘膜功能指标CBF的方法，并探讨MC与CBF之间的关系。采用高速数码摄像机拍摄了大鼠鼻中隔切除后纤毛跳动的序列图像。CBF（每秒跳动数，赫兹）是根据一系列图像中特定位置对比度值的周期性变化来确定的。对照条件下CBF为8.49+/-0.38Hz，这与培养的人鼻上皮细胞和大鼠气管细胞的值相似。-肾上腺素能和胆碱能拮抗剂降低CBF，而-肾上腺素能激动剂和乙酰胆碱增加CBF。这些结果与我们之前研究中对MC的观察结果相似。结果发现CBF与MC呈显著线性相关，表明MC受CBF的直接调控，该评价体系可定量测定鼻粘膜纤毛功能。
Mucociliary clearance (MC) is an important factor in determining nasal drug absorption and the ciliary beat of ciliated epithelial cells of the nasal mucosa is the driving force of MC. However, the relationship between MC and ciliary beat frequency (CBF) is still ambiguous. The purpose of this study was to establish an evaluation method of CBF as an index of mucociliary function and examine the relationship between MC and CBF. A sequence of images of ciliary beating of an excised rat nasal septum was captured using a high-speed digital video camera. CBF (beats per second, Hz) was determined from periodic changes in the contrast value of a specific location in a sequence of images. CBF under control conditions was 8.49+/-0.38Hz, which is similar to values reported for cultured human nasal epithelial cells and rat tracheal cells. beta-Adrenergic and cholinergic antagonists decreased CBF, while beta-adrenergic agonists and acetylcholine increased CBF. These results were similar with those observed for MC in our previous study. It was found that CBFs were significantly and linearly correlated with MC, indicating that MC is directly regulated by CBF and that this evaluation system allows the quantitative determination of nasal mucociliary function.