Apoptosis during inner ear development in human and mouse embryos: an analysis by computer-assisted three-dimensional reconstruction
Apoptosis during inner ear development in human and mouse embryos: an analysis by computer-assisted three-dimensional reconstruction
复制标题
DOI：
10.1007/s004290050255
复制
发表时间：
1999-07-01
期刊：
影响因子：
--
通讯作者：
Otani, H
中科院分区：
文献类型：
--
作者：
Nishikori, T;Hatta, T;Otani, H
通过计算机辅助的连续组织切片的三维重建和TUNEL方法，系统地分析了人（卡内基阶段14至21，约5至8周的妊娠）和小鼠（妊娠10.5至14天）胚胎的发育中内耳组织中的细胞凋亡。形态发生事件，如折叠之间的椭圆囊部分和内淋巴管，球囊与耳蜗的交界处的收缩和折叠的前庭部分，形成半圆管伴随着局部分布的细胞凋亡。细胞凋亡还与第八脑神经感觉神经节的耳蜗和前庭上皮的神经支配以及耳上皮向感觉上皮的分化有关。这些结果表明，细胞凋亡在内耳发育中起着重要作用。
Apoptosis in the developing inner ear tissue of human (Carnegie stage 14 to 21, approximately 5 to 8 weeks of gestation) and mouse (10.5 to 14 days of gestation) embryos was systematically analyzed by a computer-assisted three-dimensional reconstruction of the serial histological sections and by the TUNEL method. Morphogenetic events such as folding between the utricular portion and endolymphatic duct, constriction of the junction of the saccule with the cochlea and folding of the vestibular portion to form the semicircular ducts were accompanied by a localized distribution of apoptosis. The apoptosis was also related to the innervation of the cochlear and vestibular epithelia from the sensory ganglion of the eighth cranial nerve and the differentiation of the otic epithelia into the sensory epithelia. These results suggest that apoptosis plays an important role in the development of the inner ear.