Gene expression profiling with microarray and SAGE identifies PLUNC as a marker for hepatoid adenocarcinoma of the stomach
Gene expression profiling with microarray and SAGE identifies PLUNC as a marker for hepatoid adenocarcinoma of the stomach
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DOI：
10.1038/modpathol.3801050
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发表时间：
2008-04-01
期刊：
影响因子：
7.5
通讯作者：
Yasui, Wataru
中科院分区：
文献类型：
--
作者：
Sentani, Kazuhiro;Oue, Naohide;Yasui, Wataru
胃癌是世界上最常见的恶性肿瘤之一。在这项研究中，我们通过比较基因表达谱和基因芯片的序列分析来筛选胃癌中表达上调的基因，并鉴定了腭、肺和鼻上皮癌相关蛋白（PLUNC）基因。140例胃癌患者的PLUNC免疫染色显示，在胃肝样腺癌中，有较强的、广泛的PLUNC染色，而在常规胃癌中，只有7%的患者出现了局灶性的PLUNC免疫染色。胃肝样腺癌是一种肝外肿瘤，其形态学特征与肝细胞癌相似。为探讨PLUNC免疫染色在胃肝样腺癌诊断中的应用价值，我们对6例胃肝样腺癌（6例原发肿瘤和2例肝转移灶）进行了进一步的研究。6例原发性肝样腺癌均可见PLUNC染色。原发性肿瘤的肝样腺癌和管状/乳头状腺癌成分均可见到PLUNC染色，尽管PLUNC染色优先局限于管状/乳头状腺癌成分。两例肝转移灶均检测到PLUNC染色。52例原发性肝细胞癌、正常成人及胎儿肝脏均未见PLUNC染色。提示PLUNC是区分胃肝样腺癌与原发性肝细胞癌的新标志物。
Gastric cancer is one of the most common malignancies worldwide. In this study, we screened for genes upregulated in gastric cancer by comparing gene expression profiles from serial analysis of gene expression and microarray and identified the palate, lung, and nasal epithelium carcinoma-associated protein (PLUNC) gene. Immunostaining for PLUNC in 140 gastric cancer cases revealed strong and extensive staining of PLUNC in hepatoid adenocarcinoma of the stomach, whereas 7% of conventional gastric cancer cases showed focal immunostaining of PLUNC. Gastric hepatoid adenocarcinoma is an extrahepatic tumor characterized by morphologic similarities to hepatocellular carcinoma. To investigate the utility of PLUNC immunostaining in the diagnosis of gastric hepatoid adenocarcinoma, six cases of gastric hepatoid adenocarcinoma (six primary tumors and two associated liver metastases) were studied further. PLUNC staining was observed in all six primary hepatoid adenocarcinomas. PLUNC staining was observed in both the hepatoid adenocarcinoma and tubular/papillary adenocarcinoma components of primary tumors, although PLUNC staining was preferentially localized in tubular/papillary adenocarcinoma components. Staining of PLUNC was also detected in both liver metastases. PLUNC staining was not observed in 52 cases of primary hepatocellular carcinoma or in normal adult or fetal liver. These results indicate that PLUNC is a novel marker that distinguishes gastric hepatoid adenocarcinoma from primary hepatocellular carcinoma.