Grafted Human iPS Cell-Derived Oligodendrocyte Precursor Cells Contribute to Robust Remyelination of Demyelinated Axons after Spinal Cord Injury.
Grafted Human iPS Cell-Derived Oligodendrocyte Precursor Cells Contribute to Robust Remyelination of Demyelinated Axons after Spinal Cord Injury.
复制标题
接枝的人IPS细胞衍生的少突胶质细胞前体细胞有助于脊髓损伤后脱髓鞘轴突的稳健再生。
DOI：
10.1016/j.stemcr.2015.11.013
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发表时间：
2016-01-12
影响因子：
5.9
通讯作者：
Okano H
中科院分区：
文献类型：
--
作者：
Kawabata S;Takano M;Numasawa-Kuroiwa Y;Itakura G;Kobayashi Y;Nishiyama Y;Sugai K;Nishimura S;Iwai H;Isoda M;Shibata S;Kohyama J;Iwanami A;Toyama Y;Matsumoto M;Nakamura M;Okano H
小鼠和人诱导的多能干细胞衍生的神经干/祖细胞（iPSC-NS/PC）在移植到啮齿动物和灵长类动物的损伤脊髓中后促进功能恢复。尽管备用脱髓鞘轴突的髓鞘再生是损伤脊髓再生的关键机制，但人iPSC-NS/PC在体外和体内均主要分化为神经元。因此，我们利用我们最近开发的协议，以获得人诱导的多能干细胞来源的少突胶质细胞前体细胞富集的神经干/祖细胞，并报告这些细胞移植在脊髓损伤（SCI）模型的好处。我们描述了这种方法如何有助于脱髓鞘轴突的髓鞘再生，并促进SCI后的功能恢复。hiPSC-OPC富集NS/PC体内分化为成熟少突胶质细胞移植的hiPSC-OPC衍生的成熟少突胶质细胞有助于髓鞘再生移植的hiPSC-OPC富集NS/PC增强SCI后的功能恢复移植的hiPSC-OPC富集NS/PC促进宿主神经元的轴突生长在这篇文章中，Okano及其同事表明，移植人类诱导多能干细胞来源的少突胶质细胞前体细胞-富集的神经干/祖细胞，描述了这种方法如何有助于脱髓鞘轴突的稳健髓鞘再生，并促进脊髓损伤后的功能恢复。
Murine- and human-induced pluripotent stem cell-derived neural stem/progenitor cells (iPSC-NS/PCs) promote functional recovery following transplantation into the injured spinal cord in rodents and primates. Although remyelination of spared demyelinated axons is a critical mechanism in the regeneration of the injured spinal cord, human iPSC-NS/PCs predominantly differentiate into neurons both in vitro and in vivo. We therefore took advantage of our recently developed protocol to obtain human-induced pluripotent stem cell-derived oligodendrocyte precursor cell-enriched neural stem/progenitor cells and report the benefits of transplanting these cells in a spinal cord injury (SCI) model. We describe how this approach contributes to the robust remyelination of demyelinated axons and facilitates functional recovery after SCI. hiPSC-OPC-enriched NS/PCs differentiate into mature oligodendrocytes in vivo Grafted hiPSC-OPCs-derived mature oligodendrocytes contribute to remyelination Transplanted hiPSC-OPC-enriched NS/PCs enhance functional recovery following SCI Transplanted hiPSC-OPC-enriched NS/PCs promote axonal growth of host neurons In this article, Okano and colleagues show that the benefits of transplanting human-induced pluripotent stem cell-derived oligodendrocyte precursor cell-enriched neural stem/progenitor cells, describing how this approach contributes to the robust remyelination of demyelinated axons and facilitates functional recovery after spinal cord injury.