The microvasculature in cutaneous wound healing in the female red duroc pig is similar to that in human hypertrophic scars and different from that in the female Yorkshire pig
The microvasculature in cutaneous wound healing in the female red duroc pig is similar to that in human hypertrophic scars and different from that in the female Yorkshire pig
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DOI：
10.1097/bcr.0b013e318053dafe
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发表时间：
2007-05-01
影响因子：
1.4
通讯作者：
Engrav, Loren H.
中科院分区：
文献类型：
--
作者：
Xie, Youfu;Zhu, Kathy Q.;Engrav, Loren H.
雌性红色杜洛克猪被发现是一种有前途的增生性疤痕模型。雌性约克夏猪已被证明以一种非常不同的方式愈合，更类似于人类正常营养的疤痕。鉴于这些观察结果，我们研究了人类肥厚性疤痕以及雌性杜洛克猪和约克夏猪疤痕中的微血管密度，这是伤口愈合的一个重要方面。我们研究了未受伤皮肤的微血管密度； 6个月或更短、7至12个月和12个月以上的增生性疤痕； 3周、3个月和5个月时的雌性杜洛克组织；和类似的约克郡组织，包括未受伤的皮肤以及浅层和深层伤口。抗因子VIII相关抗原用于标记内皮细胞。微血管密度的计算评估用于量化微血管系统。在人体增生性疤痕中，微血管急剧增多，且微血管密度和面积显着升高。我们在深度受伤后 5 个月的杜洛克组织中发现了类似的结果。相比之下，我们发现约克郡组织中的微脉管系统少得多，并且在 5 个月时，这些值已恢复正常。这项微血管密度的定量研究进一步验证了雌性杜洛克猪作为增生性疤痕动物模型以及雌性约克夏猪作为对照。
The female red Duroc pig has been found to be a promising model of hypertrophic scarring. The female Yorkshire pig has been demonstrated to heal in a very different manner, more resembling human normotrophic scarring. Given these observations, we studied microvessel density, an important aspect of wound healing, in human hypertrophic scars and the scars of the female Duroc and Yorkshire pigs. We studied microvessel density in uninjured skin; hypertrophic scars at 6 months or less, 7 to 12, and longer than 12 months; female Duroc tissues at 3 weeks and 3 and 5 months; and similar Yorkshire tissue, including uninjured skin and shallow and deep wounds. Antifactor VIII-related antigen was used to mark the endothelial cells. Computed assessment of microvessel density was used to quantify the microvasculature. In human hypertrophic scars, the microvessels were increased dramatically, and microvessel density and area were significantly elevated. We found similar results in the Duroc tissues at 5 months after deep wounding. In contrast, we found far less microvasculature and, at 5 months, the values had returned to normal in the Yorkshire tissues. This quantitative study of microvessel density further validates the female Duroc pig as an animal model of hypertrophic scarring and the female Yorkshire pig as a control.