Lack of stearoyl-CoA desaturase-1 function induces a palmitoyl-CoA Delta6 desaturase and represses the stearoyl-CoA desaturase-3 gene in the preputial glands of the mouse.
Lack of stearoyl-CoA desaturase-1 function induces a palmitoyl-CoA Delta6 desaturase and represses the stearoyl-CoA desaturase-3 gene in the preputial glands of the mouse.
复制标题
缺乏硬脂酰辅酶 A 去饱和酶 1 功能会诱导小鼠包皮腺中的棕榈酰辅酶 A Delta6 去饱和酶并抑制硬脂酰辅酶 A 去饱和酶 3 基因。
作者：
M. Miyazaki;F. E. Gomez;J. Ntambi
小鼠包皮腺（PG）是一种特殊的皮脂腺结构，富含蜡酯、甘油三酯和烷基-2，3-二酰甘油。我们发现小鼠PG表达Delta 9硬脂酰辅酶A去饱和酶的三种基因亚型（SCD 1、SCD 2和SCD 3），该酶主要催化单不饱和脂肪酸的生物合成，C16：1 n-7和C18：1 n-9。然而，在SCD 1同种型（SCD 1（-/-））中具有靶向破坏的小鼠在PG中具有不可检测的SCD 3 mRNA表达，而SCD 2同种型的表达没有改变。C16：1 n-7的水平降低超过70%，而C18：1 n-9的水平降低28%。PG的C16：1 n-10（Delta 6十六碳烯酸）异构体和主要脂肪酸的含量增加了2倍以上，主要是在SCD 1（-/-）小鼠的蜡酯部分中。我们证明，增加C16：1 n-10是由于诱导一个特定的棕榈酰辅酶A Delta 6去饱和酶活性。对SCD 1（-/-）小鼠给予替吉奥诱导了SCD 3 mRNA表达，并导致16：0-CoA的Delta 9去饱和增加，但未导致18：0-CoA的Delta 9去饱和增加。这些观察结果表明，SCD 1功能的丧失改变了SCD 3的表达，并首次揭示了哺乳动物中棕榈酰-CoA Delta 6去饱和酶的存在和调节。
The mouse preputial gland (PG), a specialized sebaceous structure, is rich in wax esters, triglycerides, and alkyl-2,3-diacylglycerol. We have found that the mouse PG expresses the three gene isoforms (SCD1, SCD2, and SCD3) of the Delta9 stearoyl-CoA desaturase enzyme that catalyzes the biosynthesis of monounsaturated fatty acids mainly, C16:1n-7 and C18:1n-9. However, mice with a targeted disruption in the SCD1 isoform (SCD1(-/-)) have undetectable SCD3 mRNA expression in the PG while the expression of SCD2 isoform was not altered. The levels of C16:1n-7 were reduced by greater than 70% while that of C18:1n-9 were reduced by 28%. The content of the C16:1n-10 (Delta6 hexadecenoic acid) isomer and a major fatty acid of the PG was increased by greater than 2-fold, mainly in the wax ester fraction of the SCD1(-/-) mouse. We demonstrate that the increase in C16:1n-10 is due to induction of a specific palmitoyl-CoA Delta6 desaturase activity. Testosterone administration to the SCD1(-/-) mouse induced SCD3 mRNA expression and resulted in an increase in the Delta9 desaturation of 16:0-CoA, but not of 18:0-CoA. These observations demonstrate that loss of SCD1 function alters the expression of SCD3 and reveal for the first time the presence and regulation of a palmitoyl-CoA Delta6 desaturase enzyme in mammals.
影响因子：
4.2
作者：
Miyazaki, M;Man, WC;Ntambi, JM
通讯作者：
Ntambi, JM
3T3-L1 脂肪细胞中噻唑烷二酮对硬脂酰辅酶 A 去饱和酶基因的差异调节。
影响因子：
6.5
作者：
Kim,YC;Gomez,FE;Fox,BG;Ntambi,JM
通讯作者：
Ntambi,JM