Overexpression of allograft inflammatory factor-1 promotes proliferation of vascular smooth muscle cells by cell cycle deregulation
Overexpression of allograft inflammatory factor-1 promotes proliferation of vascular smooth muscle cells by cell cycle deregulation
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DOI：
10.1161/hq0901.095566
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发表时间：
2001-09-01
影响因子：
8.7
通讯作者：
Carbone, CM
中科院分区：
文献类型：
--
作者：
Autieri, MV;Carbone, CM
同种异体移植炎症因子-1 （AIF-1）在正常动脉中不存在，但在几种动脉损伤模型的血管平滑肌细胞（VSMCs）中被诱导表达。VSMCs的增殖是许多动脉病变中新生内膜增生的主要组成部分，本研究的目的是确定AIF-1在VSMCs生长中的作用。在原代和大鼠VSMC细胞系中转染和组成性表达AIF-1可促进细胞的生长，并与AIF-1的表达量成正比。流式细胞术检测AIF-1的组成性表达导致细胞周期缩短，Western blot检测细胞周期蛋白异常表达。AIF-1过表达也允许这些细胞在血清减少的培养基中生长。综上所述，这些数据提示AIF-1可能通过其调节VSMCs生长的能力参与血管增生性疾病的进展。
Allograft inflammatory factor-1 (AIF-1) is not present in normal arteries, but its expression is induced in vascular smooth muscle cells (VSMCs) in several models of arterial injury. The proliferation of VSMCs is a major component of neointimal hyperplasia in many arteriopathies, and the purpose of this study was to determine the role of AIF-1 in the growth of VSMCs. Transfection and constitutive expression of AIF-1 in a primary and a rat VSMC Line results in enhanced growth of those cells as measured by cell number and is proportional to the amount of AIF-1 expressed. Constitutive expression of AIF-1 results in a shorter cell cycle, as measured by flow cytometry, and aberrant expression of cell cycle proteins, as determined by Western blot. AIF-1 overexpression also permits growth of these cells in serum-reduced media. Collectively, these data suggest that AIF-1 may participate in the progression of vascular proliferative disease on the basis of its ability to regulate the growth of VSMCs.