Multiplex Ligation-Dependent Probe Amplification of Uveal Melanoma: Correlation with Metastatic Death
Multiplex Ligation-Dependent Probe Amplification of Uveal Melanoma: Correlation with Metastatic Death
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DOI：
10.1167/iovs.08-3165
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发表时间：
2009-07-01
影响因子：
4.4
通讯作者：
Coupland, Sarah E.
中科院分区：
文献类型：
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作者：
Damato, Bertil;Dopierala, Justyna;Coupland, Sarah E.
目的。为了评价葡萄膜黑色素瘤的多重连接依赖探针扩增(MLPA)作为预测转移性死亡的工具。方法：收集1998年至2000年间收治的73例葡萄膜黑色素瘤患者的标本。用MLPA分析1、3、6和8号染色体上的31个座位的DNA，结果与转移死亡相关。结果患者平均年龄60.6岁，中位随访期6.2年。28例患者发生转移性死亡，与3号染色体丢失和8q获得最密切相关(COX单变量分析，P<；0.001)。6号染色体p25区域的增加与良好的生存相关(COX单因素分析，P=0.003)。通过将可疑的3号染色体丢失视为异常，并考虑到多种危险因素，如8q增加、肿瘤直径和指示高度恶性的组织学特征，提高了转移性死亡的预测。结论：葡萄膜黑色素瘤的MLPA分析预测转移性死亡，如果统计上微不足道的3号染色体丢失与8q增加以及临床分期和组织学恶性分级一起考虑的话。(投资眼科VS科学。网址：10.1167/iovs.08-3165
PURPOSE. To evaluate multiplex ligation-dependent probe amplification (MLPA) of uveal melanoma as a predictive tool for metastatic death.METHODS. Uveal melanoma specimens of 73 patients treated between 1998 and 2000 were included. DNA samples were analyzed with MLPA evaluating 31 loci on chromosomes 1, 3, 6 and 8, and the results were correlated with metastatic death.RESULTS. The patients (27 women; 46 men) had a median age of 60.6 years and a median follow-up of 6.2 years. Metastatic death occurred in 28 patients, correlating most strongly with chromosome 3 losses and gains on 8q (Cox univariate analysis, P < 0.001). Chromosome 6, region p25, gains correlated with good survival (Cox univariate analysis, P = 0.003). Prediction of metastatic death was improved by considering equivocal chromosome 3 losses as abnormal and by taking account of multiple risk factors, such as 8q gains, tumor diameter, and histologic features indicative of high-grade malignancy.CONCLUSIONS. MLPA analysis of uveal melanoma predicts metastatic death if statistically insignificant losses of chromosome 3 are considered together with gains in 8q as well as clinical stage and histologic grade of malignancy. (Invest Ophthalmol Vis Sci. 2009;50:3048-3055) DOI: 10.1167/iovs.08-3165