The mechanism of basophil histamine release induced by antigen and by the calcium ionophore A23187.
The mechanism of basophil histamine release induced by antigen and by the calcium ionophore A23187.
复制标题
抗原和钙离子载体 A23187 诱导嗜碱性粒细胞组胺释放的机制。
DOI：
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发表时间：
1975
影响因子：
4.4
通讯作者：
Lawrence M. Lichtenstein
中科院分区：
文献类型：
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作者：
Lawrence M. Lichtenstein
将钙离子载体A23187释放人嗜碱性粒细胞组胺的机制与抗原与细胞结合IgE抗体相互作用诱导的机制进行了比较。离子载体诱导组胺释放(离子。H.R.)发生在正常和过敏供者的白细胞中。它完全依赖钙；LaCl3抑制这两种离子。抗原诱导组胺释放(Ag。hr)在10- 7 m左右离子动力学。人力资源部认为这个过程没有像Ag那样的“脱敏”阶段。hr和离子载体在抗原脱敏细胞上完全活跃。药理学研究表明，二丁基环AMP和增加内源性环AMP水平的药物不抑制离子。因为它们抑制了早期的Ag。在影响微管的药物中，秋水仙碱抑制离子，D2O增强离子。在某种程度上，与它们对Ag的影响在质量上相似，但在数量上不那么显著。代谢拮抗剂2-脱氧葡萄糖抑制两个离子。人力资源与农业人力资源部门也是一样。基于这些数据和观察，离子载体预处理的细胞显示出明显的（协同）增强Ag。人力资源部，我们得出的结论是。人力资源有一个类似或相同的机制，如果Ag的后期阶段。但“短路”的循环amp相关事件Ag。号决议
The mechasism of human basophil histamine release by the calcium ionophore A23187 has been compared to that induced by the interaction of antigen with cell bound IgE antibody. Ionophore induced histamine release (Ion. H.R.) occurs with the leukocytes of both normal and allergic donors. It is completely calcium dependent; LaCl3 inhibits both Ion. H.R. and antigen induced histamine release (Ag. H.R.) at about 10-minus 7 M. The kinetics of Ion. H.R. suggest that this process has no "desensitization" phase as does Ag. H.R. and the ionophore is fully active on antigen-desensitized cells. Pharmacologic studies indicate that dibutyryl cyclic AMP and agents which increase endogenous cyclic AMP levels do not inhibit Ion. H.R. as they inhibit the early stages of Ag. H.R. Of the agents which affect microtubules, colchicine inhibits and D2O enhances Ion. H.R. in a manner which is qualitatively similar but quantitatively less marked than their effects on Ag. H.R. The metabolic antagonist 2-deoxyglucose inhibits both Ion. H.R. and Ag. H.R. in a similar fashion. Based on these data and the observation that cells pretreated with ionophore show a marked (synergistic) enhancement of Ag. H.R. we conclude that Ion. H.R. has a similar or identical mechanism to the later stages if Ag. H.R. but "short circuits" the cyclic AMP-associated events of Ag. H.R.