Selection of Borrelia burgdorferi Promoter Sequences Active During Mammalian Infection Using In Vivo Expression Technology
Selection of Borrelia burgdorferi Promoter Sequences Active During Mammalian Infection Using In Vivo Expression Technology
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DOI：
10.1007/978-1-4939-7383-5_12
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发表时间：
2018-01-01
期刊：
影响因子：
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通讯作者：
Jewett, Mollie W.
中科院分区：
文献类型：
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作者：
Adams, Philip P.;Jewett, Mollie W.
体内表达技术（IVET）已被应用于多种生物体，以确定在特定环境或生长条件下的活性启动子。在这里，我们描述的修改，采用这种全基因组筛选方法莱姆病螺旋体，莱姆病螺旋体，在活跃的小鼠感染。该技术的利用提供了对B的有价值的见解。尽管哺乳动物宿主环境中的细菌数量很低，但在感染期间，Burgdorferi转录组的表达仍然很低。
In vivo expression technology (IVET) has been applied to a variety of organisms to identify active promoters in specific environments or growth conditions of interest. Here, we describe modifications to employ this genome-wide screening method for Borrelia burgdorferi, the Lyme disease spirochete, during an active murine infection. Utilization of this technique provides valuable insights into the B. burgdorferi transcriptome during infection, despite the low bacterial numbers in the mammalian host environment.