In vivo Wnt signaling tracing through a transgenic biosensor fish reveals novel activity domains
In vivo Wnt signaling tracing through a transgenic biosensor fish reveals novel activity domains
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DOI：
10.1016/j.ydbio.2012.03.023
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发表时间：
2012-06-15
影响因子：
2.7
通讯作者：
Argenton, Francesco
中科院分区：
文献类型：
--
作者：
Moro, Enrico;Ozhan-Kizil, Gunes;Argenton, Francesco
创建的分子工具，能够解开在体内时空激活特定的细胞信号事件在细胞迁移，分化和形态发生是非常相关的发育细胞生物学。在这里，我们描述了Wnt/β-catenin信号的两个转基因报告线，命名为TCFsiam的生成，验证和应用，并表明它们是可靠的和敏感的Wnt生物传感器在体内研究。我们证明，这些线路灵敏地检测Wnt/β-连环蛋白通路的活动在几个细胞的情况下，从感觉器官心脏瓣膜图案。我们提供的证据表明，Wnt/β-连环蛋白活性参与了斑马鱼中枢神经系统血管网络的形成和维持，在该血管网络上，sox 10神经嵴衍生细胞迁移和增殖。我们最终表明，这些转基因株系允许筛选Wnt信号转导修饰化合物，组织再生评估以及评估潜在的Wnt/β-连环蛋白遗传调节剂。(C)2012 Elsevier Inc. All rights reserved.
The creation of molecular tools able to unravel in vivo spatiotemporal activation of specific cell signaling events during cell migration, differentiation and morphogenesis is of great relevance to developmental cell biology. Here, we describe the generation, validation and applications of two transgenic reporter lines for Wnt/beta-catenin signaling, named TCFsiam, and show that they are reliable and sensitive Wnt biosensors for in vivo studies. We demonstrate that these lines sensitively detect Wnt/beta-catenin pathway activity in several cellular contexts, from sensory organs to cardiac valve patterning. We provide evidence that Wnt/beta-catenin activity is involved in the formation and maintenance of the zebrafish CNS blood vessel network, on which sox10 neural crest-derived cells migrate and proliferate. We finally show that these transgenic lines allow for screening of Wnt signaling modifying compounds, tissue regeneration assessment as well as evaluation of potential Wnt/beta-catenin genetic modulators. (C) 2012 Elsevier Inc. All rights reserved.