Cellular immune responses to four doses of percutaneous bacille Calmette-Guérin in healthy adults.
Cellular immune responses to four doses of percutaneous bacille Calmette-Guérin in healthy adults.
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健康成人对四剂经皮卡介苗的细胞免疫反应。
DOI：
10.1086/515614
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
JacobsJr,DR
中科院分区：
文献类型：
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作者：
Lowry,PW;Ludwig,TS;Adams,JA;Fitzpatrick,ML;Grant,SM;Andrle,GA;Offerdahl,MR;Cho,SN;JacobsJr,DR
为探讨低剂量免疫可诱导优先Th 1细胞免疫的假说，对76名成年人进行了4种剂量的卡介苗（BCG）免疫，即极低剂量（1.6 × 105 cfu）、低剂量（3.2 × 106 cfu）、标准剂量（1.6 × 108 cfu）和高剂量（3.2 × 108 cfu）。接种极低或低剂量BCG的人中，10%在接种后8周发生迟发型超敏反应，而接种标准或高剂量BCG的人中，迟发型超敏反应发生率分别为95%和100%。淋巴增生反应仅在高剂量疫苗接种者中增加，在疫苗接种后2周达到峰值，主要针对结核分枝杆菌分泌的蛋白质，特别是抗原85复合物。仅给予标准或高剂量BCG的人在接种疫苗后16周出现分枝杆菌特异性干扰素-γ表达的显著增加。经皮BCG似乎能够诱导暂时的Th 1样免疫应答，但需要标准或更高的剂量。
To explore the hypothesis that low-dose immunization might induce preferential Th1 cell immunity, 76 adults were vaccinated with one of four doses of bacille Calmette-Guérin (BCG): The doses contained very low (1.6 × 105cfu), low (3.2 × 106cfu), standard (1.6 × 108cfu), or high (3.2 × 108cfu) levels of BCG. Delayed-type hypersensitivity responses occurred 8 weeks after vaccination in 10% of persons given very low or low doses of BCG, compared with 95% and 100% of persons given standard or high doses, respectively. Lymphoproliferative responses, which were increased only for high-dose vaccinees, peaked 2 weeks after vaccination and were directed chiefly againstMycobacterium tuberculosis-secreted proteins, particularly the antigen 85 complex. Significant increases in mycobacteria-specific interferon-γ expression were present 16 weeks after vaccination only for persons given standard or high doses of BCG. Percutaneous BCG appears capable of inducing a temporary Th1-like immune response, but standard or higher dosages are required.