ALS2CL, the novel protein highly homologous to the carboxy-terminal half of ALS2, binds to Rab5 and modulates endosome dynamics
ALS2CL, the novel protein highly homologous to the carboxy-terminal half of ALS2, binds to Rab5 and modulates endosome dynamics
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DOI：
10.1016/j.febslet.2004.07.092
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发表时间：
2004-09-24
期刊：
影响因子：
3.5
通讯作者：
Ikeda, JE
中科院分区：
文献类型：
--
作者：
Hadano, S;Otomo, A;Ikeda, JE
ALS 2是幼年隐性肌萎缩侧索硬化症（ALS 2）的致病基因产物，是小GT受体Rab 5的鸟嘌呤核苷酸交换因子。在这里，我们报告了一个新的ALS 2同源基因，ALS 2 C-末端样（ALS 2CL），它编码一个108 kD的ALS 2CL蛋白。ALS 2CL表现出特异性但相对较弱的Rab 5-GEF活性，伴随着相当强的Rab 5-结合性质。在HeLa细胞中，ALS 2CL和Rab 5A的共表达导致内体隔室的独特的微管表型，其中ALS 2CL和Rab 5A显著共定位。这些结果表明，ALS 2CL是一种新的因子调节Rab 5介导的细胞内体动力学。(C)2004年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
ALS2, the causative gene product for juvenile recessive amyotrophic lateral sclerosis (ALS2), is a guanine-nucleotide exchange factor for the small GTPase Rab5. Here, we report a novel ALS2 homologous gene, ALS2 C-terminal like (ALS2CL), which encodes a 108-kD ALS2CL protein. ALS2CL exhibited a specific but a relatively weak Rab5-GEF activity with accompanying rather strong Rab5-binding properties. In HeLa cells, co-expression of ALS2CL and Rab5A resulted in a unique tubulation phenotype of endosome compartments with significant colocalization of ALS2CL and Rab5A. These results suggest that ALS2CL is a novel factor modulating the Rab5-mediated endosome dynamics in the cells. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.