Microfluidic quantification and separation of yeast based on surface adhesion
Microfluidic quantification and separation of yeast based on surface adhesion
复制标题
基于表面粘附的酵母微流控定量和分离
DOI：
10.1039/c9lc00275h
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发表时间：
2019
期刊：
影响因子：
6.1
通讯作者：
Fuchs, Stephen M.
中科院分区：
文献类型：
--
作者：
Reinmets, Kristina;Dehkharghani, Amin;Guasto, Jeffrey S.;Fuchs, Stephen M.
真菌粘附是从感染到食品生产到生物工程的过程的基础。然而，酵母表面粘附的强大的，人口规模的定量方法是缺乏的。我们开发了一种微流控检测来区分和分离基于粘附强度的遗传相关的酵母菌株，并量化离子强度和底物疏水性对粘附的影响。这种方法将使酵母的快速筛选和分级的基础上的遗传蛋白工程，防污表面，和其他应用的主机的粘合性能。
Fungal adhesion is fundamental to processes ranging from infections to food production to bioengineering. Yet, robust, population-scale quantification methods for yeast surface adhesion are lacking. We developed a microfluidic assay to discriminate and separate genetically-related yeast strains based on adhesion strength, and to quantify effects of ionic strength and substrate hydrophobicity on adhesion. This approach will enable the rapid screening and fractionation of yeast based on adhesive properties for genetic protein engineering, anti-fouling surfaces, and a host of other applications.