Impact of molecular hydrogen treatments on the innate immune activity and survival of zebrafish (Danio rerio) challenged with Aeromonas hydrophila
Impact of molecular hydrogen treatments on the innate immune activity and survival of zebrafish (Danio rerio) challenged with Aeromonas hydrophila
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DOI：
10.1016/j.fsi.2017.05.066
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发表时间：
2017-08-01
影响因子：
4.7
通讯作者：
Zhao, Heng
中科院分区：
文献类型：
--
作者：
Hu, Zhenyu;Wu, Bingqian;Zhao, Heng
最近，据报道，分子氢对人类和啮齿动物模型的炎症具有抑制作用。本研究的目的是评估富氢水 (HRW) 对受到嗜水气杆菌攻击的斑马鱼的预防作用。我们发现，经过硬水浸泡处理后，受到细菌攻击的斑马鱼的存活率有所提高。此外，我们还发现，HRW 能够阻止斑马鱼中嗜水气单胞菌的繁殖。此外，用有效浓度的HRW处理被嗜水气单胞菌感染的斑马鱼会强烈影响介导促炎和抗炎细胞因子的基因的表达。脾、肾和肝中选定的促炎免疫反应基因（1L-1β、IL-6 和 NF-κ B）下调，抗炎细胞因子基因 (IL-10) 上调。这项研究是第一个研究HRW对感染细菌的鱼类的影响的研究，并可能为氢的抗菌作用和在水产养殖鱼类中的进一步应用提供新的线索。 (C) 2017 Elsevier Ltd. 保留所有权利。
Recently, molecular hydrogen has been reported to have a suppressive effect on inflammation in human and rodent models. The aim of this study was to evaluate the preventive effects of hydrogen-rich water (HRW) on zebrafish challenged by A. hydrophila. We have found an increased survival rate of bacteria challenged zebrafish subjected to the HRW immersion treatment. Furthermore, we have revealed that HRW was able to block multiplication of A. hydrophila in zebrafish. In addition, treatment of zebrafish infected by A. hydrophila with effective concentrations of HRW strongly affected the expression of genes mediating pro-inflammatory and anti-inflammatory cytokines. There were down-regulation of selected pro-inflammatory immune response genes (1L-1 beta, IL-6, and NF-kappa B), and up-regulation of the anti-inflammatory cytokine gene (IL-10) in the spleen, kidney, and liver. This study is the first one to investigate the effects of HRW on fish infected with bacteria, and might shed new light on hydrogen's antimicrobial effects and further application in aquaculture fish species. (C) 2017 Elsevier Ltd. All rights reserved.