Interaction landscape of membrane-protein complexes in Saccharomyces cerevisiae
Interaction landscape of membrane-protein complexes in Saccharomyces cerevisiae
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DOI：
10.1038/nature11354
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发表时间：
2012-09-27
期刊：
影响因子：
64.8
通讯作者：
Greenblatt, Jack F.
中科院分区：
文献类型：
--
作者：
Babu, Mohan;Vlasblom, James;Greenblatt, Jack F.
涉及膜蛋白（MP）的大分子组装体具有重要的生物学作用，并且是各种疾病中的主要药物靶标（1）。大规模的亲和纯化可溶性蛋白质复合物的研究已经完成了不同的模式生物，但没有全球性的MP-复合物的成员资格已被描述到目前为止。在这里，我们报告了一个完整的调查1,590推定的积分，外周和脂质锚定的MP从酿酒酵母，这是亲和纯化的存在下，非变性洗涤剂。通过串联质谱法确定共纯化蛋白质的身份，随后用于获得高置信度的物理相互作用图，包括1，726种膜蛋白-蛋白质相互作用和501种与各种细胞膜系统相关的推定异聚复合物。我们的分析揭示了意想不到的物理协会的膜生物学的真核生物和描绘的全球拓扑景观的膜相互作用。
Macromolecular assemblies involving membrane proteins (MPs) serve vital biological roles and are prime drug targets in a variety of diseases(1). Large-scale affinity purification studies of soluble-protein complexes have been accomplished for diverse model organisms, but no global characterization of MP-complex membership has been described so far. Here we report a complete survey of 1,590 putative integral, peripheral and lipid-anchored MPs from Saccharomyces cerevisiae, which were affinity purified in the presence of non-denaturing detergents. The identities of the co-purifying proteins were determined by tandem mass spectrometry and subsequently used to derive a high-confidence physical interaction map encompassing 1,726 membrane protein-protein interactions and 501 putative heteromeric complexes associated with the various cellular membrane systems. Our analysis reveals unexpected physical associations underlying the membrane biology of eukaryotes and delineates the global topological landscape of the membrane interactome.