Genotyping human and bovine isolates of Cryptosporidium parvum by polymerase chain reaction-restriction fragment length polymorphism analysis of a repetitive DNA sequence.
Genotyping human and bovine isolates of Cryptosporidium parvum by polymerase chain reaction-restriction fragment length polymorphism analysis of a repetitive DNA sequence.
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通过重复 DNA 序列的聚合酶链反应-限制性片段长度多态性分析对人类和牛分离的小隐孢子虫进行基因分型。
DOI：
10.1111/j.1574-6968.1996.tb08107.x
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发表时间：
1996
影响因子：
2.1
通讯作者：
P. Camerlynck
中科院分区：
文献类型：
--
作者：
A. Bonnin;M. Fourmaux;J. F. Dubremetz;R. Nelson;P. Gobet;Géraldine Harly;M. Buisson;D. Puygauthier;Florence Gabriel;M. Naciri;P. Camerlynck
In order to define transmission routes of cryptosporidiosis and develop markers that distinguish Cryptosporidium parvum isolates, we have identified 2 polymorphic restriction enzyme sites in a C. parvum repetitive DNA sequence. The target sequence was amplified by polymerase chain reaction from 100 to 500 oocysts and the amplified product was subjected to restriction enzyme digestion. Typing of 23 isolates showed that 10/10 calf isolates had the same profile. In contrast, 2 patterns were observed among human isolates: 7/13 displayed the calf profile, and 6/13 presented another pattern. The PCR-RFLP assay described here is a sensitive tool to distinguish C. parvum isolates.
小隐孢子虫肌动蛋白基因的分离、序列和分子核型分析。
影响因子：
1.5
作者：
Kim,K;Goozé,L;Petersen,C;Gut,J;Nelson,RG
通讯作者：
Nelson,RG