Tet1 is required for Rb phosphorylation during G1/S phase transition
Tet1 is required for Rb phosphorylation during G1/S phase transition
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G1/S 相变期间 Rb 磷酸化需要 Tet1
DOI：
10.1016/j.bbrc.2013.02.110
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发表时间：
2013-05-03
影响因子：
3.1
通讯作者：
Wu, Denglong
中科院分区：
文献类型：
--
作者：
Huang, Shengsong;Zhu, Ziqi;Wu, Denglong
DNA甲基化在许多生物学过程中起着重要作用，包括基因表达的调节、染色质构象的维持和基因组的稳定性。TET家族蛋白将5-甲基胞嘧啶（5 mC）转化为5-羟甲基胞嘧啶（5 hmC），这表明这些酶可能参与DNA去甲基化。TET 1的功能尚未在体细胞中得到很好的表征。在这里，我们表明，在NIH 3 T3细胞Tet 1的耗竭抑制细胞生长。此外，Tet 1敲除可阻断细胞周期蛋白D1在G1期的积累，抑制Rb磷酸化，从而延迟进入G1/S期。总之，这项研究表明，Tet 1是细胞增殖所必需的，这一过程是通过Rb途径介导的。(c)2013 Elsevier Inc. All rights reserved.
DNA methylation plays an important role in many biological processes, including regulation of gene expression, maintenance of chromatin conformation and genomic stability. TET-family proteins convert 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), which indicates that these enzymes may participate in DNA demethylation. The function of TET1 has not yet been well characterized in somatic cells. Here, we show that depletion of Tet1 in NIH3T3 cells inhibits cell growth. Furthermore, Tet1 knockdown blocks cyclin D1 accumulation in G1 phase, inhibits Rb phosphorylation and consequently delays entrance to G1/S phase. Taken together, this study demonstrates that Tet1 is required for cell proliferation and that this process is mediated through the Rb pathway. (c) 2013 Elsevier Inc. All rights reserved.