Salivary proteins and early childhood caries: A gel electrophoretic analysis.
Salivary proteins and early childhood caries: A gel electrophoretic analysis.
复制标题
DOI：
10.4103/0976-237x.62515
复制
发表时间：
2010-01
影响因子：
1.2
通讯作者：
Satyamoorthy K
中科院分区：
文献类型：
--
作者：
Bhalla S;Tandon S;Satyamoorthy K
儿童早期龋齿 (ECC) 是一种常见疾病，困扰着全世界大部分儿童。过去的大量研究表明，这是细菌感染的结果，也受到宿主和饮食因素的影响。当前的龋齿研究旨在确定风险因素以及可以预防或预防龋齿发展的自然口腔防御。尽管唾液是最强大的防御系统，但它仍然具有多种特性和蛋白质，其作用尚不清楚。比较患有 ECC 的儿童和无龋齿儿童的静息人类全唾液特征。背景和设计：该研究对 100 名 4 至 6 岁儿童进行了为期 9 个月的研究，分为两组：50 名患有 ECC 的儿童和 50 名无龋齿的儿童。比较两组的全唾液流速、pH、平均蛋白浓度以及十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）唾液蛋白电泳图谱。采用SPSS（11.0版）软件包进行卡方检验、Fisher精确检验和Pearson卡方检验对数据进行比较。在凝胶电泳上，两组之间存在显着差异，无龋受试者具有更多数量的富含脯氨酸的蛋白质条带，证实了该蛋白质的保护作用。在 ECC 受试者的整个唾液中观察到糖蛋白条带数量显着增加。在两组中均观察到平均蛋白质浓度与总唾液流速之间存在显着的负相关。
Early childhood caries (ECC) is a common disease process that afflicts a large proportion of the child population worldwide. Extensive research in past indicates that it is the result of bacterial infection, also influenced by host and dietary factors. Current caries research seeks to identify risk factors as well as natural oral defenses that may protect against or prevent caries development. Saliva, in spite of being the strongest defense system, still has a wide array of properties and proteins whose role is yet not clearly known. To compare the resting human whole salivary characteristics in children with ECC and those who are caries free. Settings and Design: The study was conducted over a period of 9 months in 4- to 6-year-old 100 children comprising two groups – 50 with ECC and 50 caries free. The whole salivary flow rate, pH, mean protein concentration, and the electrophoretic profile of salivary proteins by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) were compared among both groups. The SPSS (version 11.0) software package was used to conduct the chi-square, Fisher's exact and Pearson's chi-square tests to compare the data. On gel electrophoresis, there was a significant difference among both groups with caries-free subjects having a higher number of proline-rich protein bands, substantiating the protective role of this protein. A significantly higher number of glycoprotein bands were observed in the whole saliva of subjects with ECC. A significant inverse correlation between the mean protein concentration and the whole salivary flow rate was observed in both groups.