RIBOSOMAL LOCALIZATION OF THE MESSENGER-RNA IN THE 30S INITIATION COMPLEX AS REVEALED BY UV CROSS-LINKING
RIBOSOMAL LOCALIZATION OF THE MESSENGER-RNA IN THE 30S INITIATION COMPLEX AS REVEALED BY UV CROSS-LINKING
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DOI：
10.1016/0014-5793(92)81101-q
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发表时间：
1992-10-26
期刊：
影响因子：
3.5
通讯作者：
GUALERZI, CO
中科院分区：
文献类型：
--
作者：
BRANDT, R;GUALERZI, CO
由30S核糖体亚基、P-32标记的mRNA(002mRNA)、fMet-tRNA和三种起始因子组成的翻译起始复合体经过紫外光交联，通过免疫化学方法和核酸杂交技术确定结合的mRNA的蛋白质和rRNA邻居。该基因与16S rRNA的418-615位核苷酸以及核糖体蛋白S1和S21(主要靶点)、S3、S10、S12和S14有一定程度的交联性，与S2、S7、S13、S18和S19也有少量的交联性。
Translation initiation complexes consisting of 30S ribosomal subunits, P-32-labelled mRNA (002 mRNA), fMet-tRNA and the three initiation factors were subjected to UV-crosslinking to determine the protein and rRNA neighbors of the bound mRNA by immunochemical methods and by nucleic acid hybridization techniques. The mRNA was found to be crosslinked to a specific region of the 16S rRNA spanning from nucleotide 418 to 615 and to ribosomal proteins S1 and S21 (the main targets), S3, S10, S12 and S14; a low level of crosslinking was also detected with S2, S7, S13, S18 and S19.